Han Jingjing, Sun Yuena, Song Weihua, Xu Tianjun
Laboratory of Fish Biogenetics & Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, China.
Laboratory of Fish Biogenetics & Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, China.
Dev Comp Immunol. 2017 Mar;68:79-86. doi: 10.1016/j.dci.2016.11.021. Epub 2016 Nov 25.
MicroRNAs (miRNAs) are endogenous small non-coding RNAs that participate in diverse biological processes via degrading the target mRNAs or repressing translation. In this study, the regulation of miRNA to the RLR (RIG-I-like receptor) signaling pathway by degrading the target mRNAs was researched in miiuy croaker. MDA5, a microRNA-145-5p (miR-145-5p) putative target gene, was predicted by bioinformatics, and the target sites from the 3'untranslated region of MDA5 transcripts were confirmed using luciferase reporter assays. Pre-miR-145 was more effective in inhibiting MDA5 than miR-145-5p mimic, and the effect was dose- and time-dependent. The expression patterns of miR-145-5p and MDA5 were analyzed in liver and kidney from miiuy croaker. Results implied that miR-145-5p may function via degrading the MDA5 mRNAs, thereby regulating the RLR signaling pathway. Studies on miR-145-5p will enrich knowledge of its functions in immune response regulation in fish, as well as offer a basis for regulatory networks that are composed of numerous miRNAs.
微小RNA（miRNA）是内源性小非编码RNA，通过降解靶标mRNA或抑制翻译参与多种生物学过程。在本研究中，在大黄鱼中研究了miRNA通过降解靶标mRNA对RLR（视黄酸诱导基因I样受体）信号通路的调控。通过生物信息学预测了MDA5这一微小RNA-145-5p（miR-145-5p）的假定靶基因，并使用荧光素酶报告基因检测法确认了MDA5转录本3'非翻译区的靶位点。Pre-miR-145在抑制MDA5方面比miR-145-5p模拟物更有效，且该效应呈剂量和时间依赖性。分析了大黄鱼肝脏和肾脏中miR-145-5p和MDA5的表达模式。结果表明，miR-145-5p可能通过降解MDA5 mRNA发挥作用，从而调控RLR信号通路。对miR-145-5p的研究将丰富其在鱼类免疫反应调控中功能的知识，也为众多miRNA组成的调控网络提供依据。
