Scheving L A, Tsai T H, Cornett L E, Feuers R J, Scheving L E
Department of Medicine, Stanford University, California 94305.
Anat Rec. 1989 Aug;224(4):459-65. doi: 10.1002/ar.1092240402.
The liver undergoes a biochemical and morphological circadian transformation. In this paper, we document circadian variation in the binding parameters of the hepatic epidermal growth factor receptor (EGFR). Liver membranes were prepared from ad libitum fed or fasted male CD2F1 mice killed at different circadian phases at 4 h intervals. Bmax (maximum binding) and Kd (dissociation constant) varied in a rhythmic fashion. The range of change for Bmax along the 24 h time scale was 423%. For Kd, it was 162%. Both peaked late in the dark span, and decreased late in the light span. Fasting and EGF treatment reduced Bmax and the amplitude of circadian variation.
肝脏会经历生化和形态学上的昼夜节律转变。在本文中，我们记录了肝脏表皮生长因子受体（EGFR）结合参数的昼夜节律变化。从自由进食或禁食的雄性CD2F1小鼠制备肝膜，这些小鼠在不同的昼夜节律阶段每隔4小时处死一批。最大结合量（Bmax）和解离常数（Kd）呈节律性变化。在24小时时间尺度上，Bmax的变化范围为423%。Kd的变化范围为162%。两者均在黑暗期后期达到峰值，并在光照期后期下降。禁食和表皮生长因子（EGF）处理降低了Bmax和昼夜节律变化的幅度。
