Seidman J G, Leder A, Edgell M H, Polsky F, Tilghman S M, Tiemeier D C, Leder P
Proc Natl Acad Sci U S A. 1978 Aug;75(8):3881-5. doi: 10.1073/pnas.75.8.3881.
We have identified at least six EcoRI fragments of mouse DNA that encode variable-region gene sequences closely related to the mouse kappa light chain, MOPC-149. Two of these fragments have been cloned, and the entire nucleotide sequence of the variable-region genes encoded on each has been determined. Both genes encode closely related variable-region sequences extending from codon position 1 through position 97. Neither fragment encodes a constant-region sequence. Although both genes are closely related, they differ from one another and from the sequence expressed in the MOPC-149 cell from which they were cloned. These few differences cluster within the complementarity-determining regions although several occur in framework sequences as well. We therefore conclude that an antibody-producing cell contains genetic information corresponding to its expressed sequence and several other closely related but silent sequences. These initial results raise the possibility that similar sets of genes might exist corresponding to each of the many subgroups already identified among mouse kappa light chains. If true, this would further suggest that the mouse genome might be rich enough in variable-region genes so as to encode a major portion of the variable-region repertoire.
我们已经鉴定出小鼠DNA的至少六个EcoRI片段，它们编码与小鼠κ轻链MOPC-149密切相关的可变区基因序列。其中两个片段已被克隆，并确定了每个片段上编码的可变区基因的完整核苷酸序列。这两个基因都编码从密码子位置1到97的密切相关的可变区序列。两个片段均未编码恒定区序列。虽然这两个基因密切相关，但它们彼此不同，也与从中克隆它们的MOPC-149细胞中表达的序列不同。这些少数差异集中在互补决定区内，尽管也有一些出现在框架序列中。因此，我们得出结论，产生抗体的细胞包含与其表达序列以及其他几个密切相关但沉默的序列相对应的遗传信息。这些初步结果增加了一种可能性，即可能存在与小鼠κ轻链中已经鉴定出的许多亚组中的每一个相对应的类似基因集。如果是这样，这将进一步表明小鼠基因组中的可变区基因可能足够丰富，从而能够编码可变区库的主要部分。
