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免疫球蛋白可变κ基因多拷贝在黏粒载体中的克隆

Cloning of multiple copies of immunoglobulin variable kappa genes in cosmid vectors.

作者信息

Cattaneo R, Gorski J, Mach B

出版信息

Nucleic Acids Res. 1981 Jun 25;9(12):2777-90. doi: 10.1093/nar/9.12.2777.

DOI:10.1093/nar/9.12.2777
PMID:6269060
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC326892/
Abstract

The possibility of cloning large segments of DNA in cosmid vectors offers distinct advantages, in particular for the study of multigene families. Large size fragments of mouse embryo DNA were successfully cloned in the cosmid pHC 79. Twelve recombinants hybridizing specifically to an immunoglobulin kappa chain variable region probe were identified. In 9 of these recombinants, the size of the insert ranges from 30 to 43 kilobases. Factors affecting the cloning efficiency of a complex mammalian genome in cosmids were studied. The stability of these recombinant cosmids and the preparation of recombinant cosmid DNA are also discussed.

摘要

利用黏粒载体克隆大片段DNA的可能性具有明显优势，尤其对于多基因家族的研究而言。小鼠胚胎DNA的大片段已成功克隆到黏粒pHC 79中。鉴定出了十二个与免疫球蛋白κ链可变区探针特异性杂交的重组体。在其中九个重组体中，插入片段的大小在30至43千碱基之间。研究了影响复杂哺乳动物基因组在黏粒中克隆效率的因素。还讨论了这些重组黏粒的稳定性以及重组黏粒DNA的制备。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3838/326892/5e405a519dac/nar00405-0147-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3838/326892/a498e63eac3f/nar00405-0142-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3838/326892/ed86aad5ef63/nar00405-0145-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3838/326892/51cd3c3006ad/nar00405-0146-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3838/326892/5e405a519dac/nar00405-0147-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3838/326892/a498e63eac3f/nar00405-0142-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3838/326892/ed86aad5ef63/nar00405-0145-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3838/326892/51cd3c3006ad/nar00405-0146-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3838/326892/5e405a519dac/nar00405-0147-a.jpg

相似文献

1
Cloning of multiple copies of immunoglobulin variable kappa genes in cosmid vectors.免疫球蛋白可变κ基因多拷贝在黏粒载体中的克隆
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2
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引用本文的文献

1
Patents and literature.专利与文献。
Appl Biochem Biotechnol. 1981 Dec;6(4):339-51. doi: 10.1007/BF02798284.

本文引用的文献

1
A small cosmid for efficient cloning of large DNA fragments.一种用于高效克隆大片段DNA的小型黏粒。
Gene. 1980 Nov;11(3-4):291-8. doi: 10.1016/0378-1119(80)90069-4.
2
A new high-capacity cosmid vector and its use.一种新型高容量黏粒载体及其应用。
Gene. 1980 Nov;11(3-4):271-82. doi: 10.1016/0378-1119(80)90067-0.
3
Stimulation by cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein relaxation complex.环磷酸腺苷对质粒脱氧核糖核酸复制的刺激作用以及对质粒脱氧核糖核酸 - 蛋白质松弛复合物的分解代谢物阻遏作用。
J Bacteriol. 1973 May;114(2):577-91. doi: 10.1128/jb.114.2.577-591.1973.
4
Isolation of high-molecular-weight DNA from mammalian cells.从哺乳动物细胞中分离高分子量DNA。
Eur J Biochem. 1973 Jul 2;36(1):32-8. doi: 10.1111/j.1432-1033.1973.tb02881.x.
5
Detection of specific sequences among DNA fragments separated by gel electrophoresis.在通过凝胶电泳分离的DNA片段中检测特定序列。
J Mol Biol. 1975 Nov 5;98(3):503-17. doi: 10.1016/s0022-2836(75)80083-0.
6
Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.菌落杂交：一种分离含有特定基因的克隆DNA的方法。
Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5. doi: 10.1073/pnas.72.10.3961.
7
Charon phages: safer derivatives of bacteriophage lambda for DNA cloning.卡戎噬菌体：用于DNA克隆的λ噬菌体更安全的衍生物。
Science. 1977 Apr 8;196(4286):161-9. doi: 10.1126/science.847462.
8
Evidence for somatic rearrangement of immunoglobulin genes coding for variable and constant regions.编码可变区和恒定区的免疫球蛋白基因体细胞重排的证据。
Proc Natl Acad Sci U S A. 1976 Oct;73(10):3628-32. doi: 10.1073/pnas.73.10.3628.
9
The rabbit beta-globin gene contains a large large insert in the coding sequence.兔β-珠蛋白基因在编码序列中含有一个大的插入片段。
Cell. 1977 Dec;12(4):1097-108. doi: 10.1016/0092-8674(77)90172-6.
10
Cosmids: a type of plasmid gene-cloning vector that is packageable in vitro in bacteriophage lambda heads.黏粒：一种质粒基因克隆载体，可在体外包装到噬菌体λ头部。
Proc Natl Acad Sci U S A. 1978 Sep;75(9):4242-6. doi: 10.1073/pnas.75.9.4242.