Department of Biosciences, School of Science and Engineering, Teikyo University, Utsunomiya, Tochigi, 320-8551, Japan.
Division of Integrated Science and Engineering, Teikyo University Graduate School of Science and Engineering, Utsunomiya, Tochigi, 320-8551, Japan.
Sci Rep. 2016 Nov 30;6:38111. doi: 10.1038/srep38111.
Histone gene expression is regulated in a cell cycle-dependent manner, with a peak at S phase, which is crucial for cell division and genome integrity. However, the detailed mechanisms by which expression of histone genes are tightly regulated remain largely unknown. Fission yeast Ams2, a GATA-type zinc finger motif-containing factor, is required for activation of S phase-specific core histone gene transcription. Here we report the molecular characterisation of Ams2. We show that the zinc finger motif in Ams2 is necessary to bind the histone gene promoter region and to activate histone gene transcription. An N-terminal region of Ams2 acts as a self-interaction domain. Intriguingly, N-terminally truncated Ams2 binds to the histone gene promoters, but does not fully activate histone gene transcription. These observations imply that Ams2 self-interactions are required for efficient core histone gene transcription. Moreover, we show that Ams2 interacts with Teb1, which itself binds to the core histone gene promoters. We discuss the relationships between Ams2 domains and efficient transcription of the core histone genes in fission yeast.
组蛋白基因的表达在细胞周期中受到调控，在 S 期达到高峰，这对细胞分裂和基因组完整性至关重要。然而，组蛋白基因表达受严格调控的详细机制在很大程度上仍不清楚。裂殖酵母 Ams2 是一种含有 GATA 型锌指结构域的因子，它是激活 S 期特异性核心组蛋白基因转录所必需的。在这里，我们报告了 Ams2 的分子特征。我们表明，Ams2 中的锌指结构域对于结合组蛋白基因启动子区域和激活组蛋白基因转录是必需的。Ams2 的 N 端区域充当自我相互作用结构域。有趣的是，N 端截断的 Ams2 结合到组蛋白基因启动子上，但不能完全激活组蛋白基因转录。这些观察结果表明，Ams2 自身相互作用对于有效的核心组蛋白基因转录是必需的。此外，我们表明 Ams2 与 Teb1 相互作用，而 Teb1 本身结合到核心组蛋白基因启动子上。我们讨论了 Ams2 结构域与裂殖酵母中核心组蛋白基因高效转录之间的关系。
