神经元产生的一种因子可诱导培养的肌肉细胞上未聚集的乙酰胆碱受体发生部分固定。
A factor from neurons induces partial immobilization of nonclustered acetylcholine receptors on cultured muscle cells.
作者信息
Axelrod D, Bauer H C, Stya M, Christian C N
出版信息
J Cell Biol. 1981 Feb;88(2):459-62. doi: 10.1083/jcb.88.2.459.
Abstract
A factor or factors released by cultured NG108-15 neuroblastoma X glioma hybrid cells and added to the medium of rat myotube primary cultures was found to immobilize some of the previously mobile acetylcholine receptors in the myotube membrane. Partial receptor immobilization occurred within 3 h after the beginning of treatment with the NG108-15-conditioned medium factor and persisted for at least 24 h of continuous treatment. A similarly derived conditioned medium concentrate from the non-neuronal parent glioma cell line did not immobilize receptors, relative to untreated controls. Acetylcholine receptors were visualized by fluorescent alpha-bungarotoxin and their lateral motion was observed by the technique of fluorescence photobleaching recovery.
摘要
研究发现，培养的NG108 - 15神经母细胞瘤X胶质瘤杂交细胞释放的一种或多种因子，添加到大鼠肌管原代培养物的培养基中后，可使肌管膜中一些先前可移动的乙酰胆碱受体固定化。在用NG108 - 15条件培养基因子处理开始后的3小时内，部分受体发生固定化，并在持续处理至少24小时后仍持续存在。相对于未处理的对照，来自非神经元亲本胶质瘤细胞系的类似衍生的条件培养基浓缩物不会使受体固定化。通过荧光α - 银环蛇毒素使乙酰胆碱受体可视化，并通过荧光光漂白恢复技术观察其侧向运动。
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本文引用的文献
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