Kaparullina Elena N, Trotsenko Yuri A, Doronina Nina V
G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Moscow region 142290, Russian Federation.
Int J Syst Evol Microbiol. 2017 Feb;67(2):425-431. doi: 10.1099/ijsem.0.001646. Epub 2017 Feb 24.
Three strains of obligately methylotrophic Betaproteobacteria (ZT, SP and M3) with the ribulose monophosphate pathway of C1 assimilation are described. The isolates were strictly aerobic, Gram-stain-negative, asporogenous, motile (strains ZT and M3) or non-motile (strain SP) rods that multiplied by binary fisson, and were mesophilic and neutrophilic. All three strains utilized methanol but only strains SP and M3 utilized methylamine as carbon and energy sources. The prevailing cellular fatty acids were straight-chain saturated C16 : 0 and unsaturated C16 : 1ω7c acids. The major ubiquinone was Q-8. The predominant phospholipids were phosphatidylethanolamine, phosphatidylglycerol and cardiolipin. Ammonia was assimilated by glutamate dehydrogenase. The DNA G+C contents of strains ZT, SP and M3 were 51.0, 52.0 and 52.0 mol% (Tm), respectively. Levels of 16S rRNA gene sequence similarity between the three strains were very high (99.9-100 %), and they shared high levels of DNA-DNA relatedness (88-98 %). Based on 16S rRNA gene sequence analysis and DNA-DNA relatedness (19-30 %) with the type strains of the genus Methylobacillus, the novel isolates ZT, SP and M3 are classified as representing a novel species of this genus, for which the name Methylobacillus methanolivorans sp. nov. is proposed. The type strain is ZT (=VKM B-3037T=JCM 31401T=CCUG 68999T).
描述了三株具有磷酸核酮糖途径进行C1同化的专性甲基营养型β-变形菌（ZT、SP和M3）。这些分离株为严格需氧、革兰氏染色阴性、不产芽孢、能动（菌株ZT和M3）或不能动（菌株SP）的杆菌，通过二分分裂繁殖，为嗜温菌和嗜中性菌。所有三株菌株都利用甲醇，但只有菌株SP和M3利用甲胺作为碳源和能源。主要的细胞脂肪酸是直链饱和C16 : 0和不饱和C16 : 1ω7c酸。主要的泛醌是Q-8。主要的磷脂是磷脂酰乙醇胺、磷脂酰甘油和心磷脂。氨通过谷氨酸脱氢酶进行同化。菌株ZT、SP和M3的DNA G+C含量分别为51.0、52.0和52.0 mol%（Tm）。这三株菌株之间的16S rRNA基因序列相似性水平非常高（99.9 - 100 %），并且它们具有高水平的DNA - DNA相关性（88 - 98 %）。基于16S rRNA基因序列分析以及与甲基芽孢杆菌属模式菌株的DNA - DNA相关性（19 - 30 %），新分离株ZT、SP和M3被分类为代表该属的一个新物种，为此提出名称为嗜甲醇甲基芽孢杆菌（Methylobacillus methanolivorans sp. nov.）。模式菌株是ZT（=VKM B - 3037T = JCM 31401T = CCUG 68999T）。
