G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences and Pushchino State University, Pushchino, Moscow region 142290, Russia.
Int J Syst Evol Microbiol. 2010 Nov;60(Pt 11):2623-2628. doi: 10.1099/ijs.0.019455-0. Epub 2009 Dec 18.
Novel yellow, obligately methylotrophic and restricted facultatively methylotrophic bacteria, respectively designated strains Ship(T) and Mim(T), with the ribulose monophosphate pathway of C(1) assimilation are described. Cells were strictly aerobic, Gram-negative, asporogenous, non-motile rods that multiply by binary fission, were mesophilic and neutrophilic and synthesized indole-3-acetic acid and exopolysaccharide. The predominant cellular fatty acids were C(16 : 0) and C(16 : 1). The major ubiquinone was Q-8. The predominant phospholipids were phosphatidylethanolamine and phosphatidylglycerol; diphosphatidylglycerol was absent. The two strains lacked α-ketoglutarate dehydrogenase and glutamate dehydrogenase. They assimilated ammonium via the glutamate cycle enzymes glutamine synthetase and glutamate synthase. The DNA G+C contents of strains Ship(T) and Mim(T) were 50.7 and 54.5 mol% (T(m)), respectively. The level of 16S rRNA gene sequence similarity between these strains was very high (99.8 %) but they shared a low level of DNA-DNA relatedness (44 %). Based on 16S rRNA gene sequence analysis and low levels of DNA-DNA relatedness with the type strains of recognized species of the genus Methylophilus (31-36 %), strains Ship(T) and Mim(T) are considered to represent novel species of the genus Methylophilus, for which the names Methylophilus flavus sp. nov. (type strain Ship(T) =DSM 23073(T) =VKM B-2547(T) =CCUG 58411(T)) and Methylophilus luteus sp. nov. (type strain Mim(T) =DSM 22949(T) =VKM B-2548(T) =CCUG 58412(T)) are proposed.
新型黄色、严格依赖甲醇和兼性依赖甲醇的细菌，分别命名为菌株 Ship（T）和 Mim（T），它们具有 C（1）同化的核酮糖单磷酸途径。细胞严格需氧，革兰氏阴性，无芽孢，不运动，通过二分分裂繁殖，中温，嗜中性，合成吲哚-3-乙酸和胞外多糖。主要细胞脂肪酸为 C（16：0）和 C（16：1）。主要的醌为 Q-8。主要磷脂为磷脂酰乙醇胺和磷脂酰甘油；双磷脂酰甘油不存在。两株菌均缺乏α-酮戊二酸脱氢酶和谷氨酸脱氢酶。它们通过谷氨酸循环酶谷氨酰胺合成酶和谷氨酸合酶同化铵。菌株 Ship（T）和 Mim（T）的 DNA G+C 含量分别为 50.7 和 54.5 mol%（T（m））。这两株菌的 16S rRNA 基因序列相似度非常高（99.8%），但它们的 DNA-DNA 同源性较低（44%）。基于 16S rRNA 基因序列分析和与已识别的甲基杆菌属种的 DNA-DNA 同源性较低（31-36%），菌株 Ship（T）和 Mim（T）被认为代表甲基杆菌属的新型种，分别命名为黄色甲基杆菌（Methylophilus flavus）sp. nov.（模式菌株 Ship（T）=DSM 23073（T）=VKM B-2547（T）=CCUG 58411（T））和黄色甲基杆菌（Methylophilus luteus）sp. nov.（模式菌株 Mim（T）=DSM 22949（T）=VKM B-2548（T）=CCUG 58412（T））。
