G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences Pushchino, Moscow region 142290, Russia.
Int J Syst Evol Microbiol. 2014 Jan;64(Pt 1):158-164. doi: 10.1099/ijs.0.055046-0. Epub 2013 Sep 18.
A newly isolated facultatively methylotrophic bacterium (strain 3t(T)) was investigated. Cells of the isolate were Gram-stain-negative, asporogenous, non-motile rods that multiplied by binary fission. The strain utilized methanol, methylamine and a variety of multicarbon compounds as carbon and energy sources. Growth occurred at pH 6.5-8.5 (optimally at 7.0-7.5) and at 10-45 °C (optimally at 30-37 °C). The major fatty acids of methanol-grown cells were C16 : 1ω7c and C16 : 0. The predominant phospholipids were phosphatidylethanolamine and phosphatidylglycerol. The major ubiquinone was Q-8. Strain 3t(T) possessed pyrroloquinoline quinone (PQQ)-linked methanol dehydrogenase and assimilated C1 units at the level of formaldehyde and CO2 via the serine cycle. The DNA G+C content of the strain was 63.6 mol% (Tm). On the basis of 16S rRNA gene sequence similarity (98.1 %) and rather low DNA-DNA relatedness (30 %) with the type strain of the type species of the genus Methyloversatilis (Methyloversatilis universalis FAM5(T)), and physiological and biochemical characteristics, the isolate was classified as a representative of a new species of the genus and named Methyloversatilis thermotolerans 3t(T) ( = VKM B-2692(T) = CCUG 61694(T) = DSM 25156(T)).
新分离的兼性甲基营养细菌（菌株 3t(T)）被研究。该分离株的细胞革兰氏阴性，无孢子，不运动的杆状，通过二分分裂繁殖。该菌株利用甲醇、甲胺和多种多碳化合物作为碳源和能源。在 pH 6.5-8.5（最佳 pH 值为 7.0-7.5）和 10-45°C（最佳温度为 30-37°C）下生长。甲醇生长细胞的主要脂肪酸为 C16 : 1ω7c 和 C16 : 0。主要的磷脂是磷脂酰乙醇胺和磷脂酰甘油。主要的泛醌是 Q-8。菌株 3t(T)具有吡咯喹啉醌（PQQ）连接的甲醇脱氢酶，并通过丝氨酸循环将 C1 单位同化至甲醛和 CO2 水平。该菌株的 DNA G+C 含量为 63.6 mol%（Tm）。基于 16S rRNA 基因序列相似性（98.1%）和与属名 Methyloversatilis 模式种的模式株（Methyloversatilis universalis FAM5(T)）的相当低的 DNA-DNA 同源性（30%），以及生理生化特征，该分离株被分类为该属的一个新种的代表，并命名为 Methyloversatilis thermotolerans 3t(T)（=VKM B-2692(T)=CCUG 61694(T)=DSM 25156(T)）。
