Zhao Qi, Li Hui-Ru, Han Qing-Qing, He Ao-Lei, Nie Cong-Yuan, Wang Suo-Min, Zhang Jin-Lin
State Key Laboratory of Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730000, PR China.
Forestry Workstation of Alxa Right Banner, Inner Mongolia Autonomous Region, Alxa 750300, Inner Mongolia, PR China.
Int J Syst Evol Microbiol. 2017 Feb;67(2):454-459. doi: 10.1099/ijsem.0.001652. Epub 2017 Feb 24.
An alkaliphilic strain designed MN-1T was isolated from a desert sand sample collected from Tengger desert, north-western China. To delineate its taxonomic position, this Gram-stain-negative, rod-shaped, strictly aerobic bacterium was subjected to a polyphasic taxonomic study. Growth was observed at temperatures from 4 to 37 °C (optimum 30-32 °C), at salinities from 0 to 2 % (optimum 1 %) and at pH from 6.5 to 12.0 (optimum 7.0-9.0). Phylogenetic analysis based on 16S rRNA gene sequencing showed that strain MN-1T was a member of the genus Altererythrobacterbut could be distinguished from recognized species of this genus. Compared to the reference strains, the novel strain was flagellated and motile by means of polar flagella. The predominant respiratory quinone was ubiquinone-10 and the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid, phosphatidylglycerol, phosphatidylcholine, one unidentified glycolipid, one unidentified phospholipid and four unidentified lipids. The predominant fatty acids were C18 : 1ω7c, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0. These chemotaxonomic traits were in agreement with the characteristics of the genus Altererythrobacter. Strain MN-1T was most closely related to Altererythrobacter xinjiangensis S3-63T (96.9 % 16S rRNA gene sequence similarity), followed by Altererythrobacter dongtanensis JM27T (96.4 %) and Altererythrobacter marinus H32T (96.1 %). The G+C content of the genomic DNA of strain MN-1T was 67.0 mol%. On the basis of data from this polyphasic taxonomic study, strain MN-1T is proposed as the type strain of a novel species of the genus Altererythrobacter, named as Altererythrobacter soli sp. nov. (=KCTC 52135T=MCCC 1K02066T).
从中国西北部腾格里沙漠采集的沙漠沙样中分离出一株嗜碱菌株MN-1T。为了确定其分类地位，对这株革兰氏染色阴性、杆状、严格需氧的细菌进行了多相分类研究。在4至37 °C（最适温度30 - 32 °C）、盐度0至2 %（最适盐度1 %）以及pH值6.5至12.0（最适pH值7.0 - 9.0）的条件下均观察到该菌株生长。基于16S rRNA基因序列的系统发育分析表明，菌株MN-1T是红杆菌属（Altererythrobacter）的成员，但可与该属已确认的物种区分开来。与参考菌株相比，该新菌株具有鞭毛，通过极生鞭毛运动。主要呼吸醌为泛醌-10，主要极性脂为双磷脂酰甘油、磷脂酰乙醇胺、鞘糖脂、磷脂酰甘油、磷脂酰胆碱、一种未鉴定的糖脂、一种未鉴定的磷脂和四种未鉴定的脂类。主要脂肪酸为C18 : 1ω7c、总和特征3（C16 : 1ω7c和/或C16 : 1ω6c）以及C16 : 0。这些化学分类特征与红杆菌属的特征一致。菌株MN-1T与新疆红杆菌（Altererythrobacter xinjiangensis）S3-63T关系最为密切（16S rRNA基因序列相似性为96.9 %），其次是东滩红杆菌（Altererythrobacter dongtanensis）JM27T（96.4 %）和海红杆菌（Altererythrobacter marinus）H32T（96.1 %）。菌株MN-1T基因组DNA的G+C含量为67.0 mol%。基于这项多相分类研究的数据，菌株MN-1T被提议作为红杆菌属一个新物种的模式菌株，命名为沙漠红杆菌（Altererythrobacter soli）新种（=KCTC 52135T = MCCC 1K02066T）。
