Yuan Nate, Zeng Yanhua, Feng Hao, Yu Zhiliang, Huang Yili
College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, PR China.
Department of Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, PR China.
Int J Syst Evol Microbiol. 2017 Sep;67(9):3655-3659. doi: 10.1099/ijsem.0.002198. Epub 2017 Sep 7.
A Gram-stain-negative, coccoid, yellow, non-motile, aerobic bacterium, designated strain S36T, was isolated from soil of the Xixi wetland in Zhejiang province, PR China. Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain S36T could represent a novel species of genus Altererythrobacter showing highest similarity to Altererythrobacter atlanticus 26DY36T (96.31 % 16S rRNA gene sequence similarity). The temperature, pH and NaCl concentration ranges for growth were 10-37 °C (optimum 32 °C), pH 5.0-10.0 (optimum pH 7.0) and 0.5-3 % (optimum 1 %, w/v), respectively. The predominant respiratory quinone of strain S36T was Q-10. The major fatty acids were C16 : 0, C17 : 1ω6c, C18 : 1ω7c and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH). The G+C content of the genomic DNA was 62.7 mol%. These data all support the affiliation of strain S36T to the genus Altererythrobacter. The polar lipids profile of strain S36T comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified phospholipids and two unidentified glycolipids. The results of physiological and biochemical tests allowed differentiation of strain S36T from other members of the genus Altererythrobacter. Therefore, strain S36T represents a novel species of the genus Altererythrobacter, for which the name Altererythrobacter xixiisoli sp. nov. is proposed; the type strain is S36T (=CGMCC 1.12804T=NBRC 110413T).
从中国浙江省西溪湿地土壤中分离出一株革兰氏阴性、球状、黄色、无运动性、需氧的细菌，命名为菌株S36T。基于16S rRNA基因序列的系统发育分析表明，菌株S36T可能代表交替赤杆菌属的一个新物种，与大西洋交替赤杆菌26DY36T的相似性最高（16S rRNA基因序列相似性为96.31%）。其生长的温度范围为10-37 °C（最适温度32 °C），pH范围为5.0-10.0（最适pH 7.0），NaCl浓度范围为0.5-3%（最适1%，w/v）。菌株S36T的主要呼吸醌为Q-10。主要脂肪酸为C16 : 0、C17 : 1ω6c、C18 : 1ω7c和特征性总和3（C16 : 1ω7c和/或异-C15 : 0 2-OH）。基因组DNA的G+C含量为62.7 mol%。这些数据均支持菌株S36T属于交替赤杆菌属。菌株S36T的极性脂质谱包括双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、两种未鉴定的磷脂和两种未鉴定的糖脂。生理生化测试结果使菌株S36T能够与交替赤杆菌属的其他成员区分开来。因此，菌株S36T代表交替赤杆菌属的一个新物种，提议将其命名为西溪交替赤杆菌（Altererythrobacter xixiisoli sp. nov.）；模式菌株为S36T（=CGMCC 1.12804T=NBRC 110413T）。
