Harvey D J
University Department of Pharmacology, Oxford, UK.
Biomed Environ Mass Spectrom. 1989 Sep;18(9):719-23. doi: 10.1002/bms.1200180912.
Human cerumen was hydrolysed with base and the constituents were examined as trimethylsilyl (TMS), methyl ester/TMS, picolinyl/TMS and nicotinate/TMS derivatives by gas chromatography and gas chromatography/mass spectrometry. A sample was also reacted with osmium tetroxide for double bond location. The major constituents were cholesterol, squalene and several series of long-chain fatty acids and alcohols. These latter compounds had chain lengths of 12-26 carbon atoms and were predominantly either straight-chain saturated or straight-chain unsaturated compounds. Saturated branched-chain acids with methyl groups predominantly on even-numbered carbon atoms were present but were less abundant. Unsaturated, branched-chain acids were also present. The major unsaturated acids contained unsaturation at the delta-6-position or were derived from these acids by chain elongation. The compounds were similar to those found in vernix caseosa. The mass spectra of picolinyl esters were, for the first time, shown to be capable of determining both the position of unsaturation and methyl branching in the same molecule.
人耳垢用碱水解，其成分通过气相色谱法和气相色谱/质谱法作为三甲基硅烷基（TMS）、甲酯/TMS、吡啶基/TMS和烟酸酯/TMS衍生物进行检测。还使一个样品与四氧化锇反应以确定双键位置。主要成分是胆固醇、角鲨烯以及几个系列的长链脂肪酸和醇。后一类化合物的链长为12至26个碳原子，主要是直链饱和或直链不饱和化合物。存在甲基主要位于偶数碳原子上的饱和支链酸，但含量较少。也存在不饱和支链酸。主要的不饱和酸在δ-6位含有不饱和键，或者是通过链延长从这些酸衍生而来。这些化合物与在胎脂中发现的化合物相似。首次表明吡啶基酯的质谱能够确定同一分子中不饱和键的位置和甲基支链。
