霍奇金淋巴瘤异常细胞的细胞遗传学和分子遗传学分析。

Cytogenetic and molecular genetic analysis of abnormal cells in Hodgkin's disease.

作者信息

Koduru P R, Offit K, Filippa D A, Lieberman P H, Jhanwar S C

机构信息

Department of Pathology, Memorial Sloan-Kettering Cancer Center, New York.

出版信息

Cancer Genet Cytogenet. 1989 Nov;43(1):109-18. doi: 10.1016/0165-4608(89)90134-9.

DOI:10.1016/0165-4608(89)90134-9

PMID:2790766

Abstract

Eleven tumors from ten patients with Hodgkin's diseases (HD) were characterized by histologic, cytogenetic, immunophenotypic, and genotypic studies. Cell surface markers for lymphocyte antigens did not show clonal excess. Five tumors showed the presence of karyotypically abnormal cells, but no common abnormalities were found. The remaining six tumors showed normal karyotypes. Ten tumors were analyzed for gene rearrangements with probes for IgJH, IgCk. IgC lambda, and TCR-beta genes. The IgJH probe detected a minor clonal population (about 5%) in one tumor with abnormal karyotype; three tumors with abnormal karyotypes showed germline genotype. In contrast, four of the six tumors with normal karyotypes showed rearrangements in IgJH (one tumor) and in C-lambda (three tumors) genes. The pattern of gene rearrangement observed in these tumors did not obey the hierarchy described in B-cell differentiation. These results suggest that B-cell lineage cannot be attributed unequivocally to the clonal populations in HD.

摘要

对10例霍奇金病（HD）患者的11个肿瘤进行了组织学、细胞遗传学、免疫表型和基因分型研究。淋巴细胞抗原的细胞表面标志物未显示克隆性过剩。5个肿瘤显示存在核型异常细胞，但未发现共同异常。其余6个肿瘤显示核型正常。用IgJH、IgCk、IgCλ和TCR-β基因探针分析了10个肿瘤的基因重排。IgJH探针在1个核型异常的肿瘤中检测到少量克隆群体（约5%）；3个核型异常的肿瘤显示种系基因型。相比之下，6个核型正常的肿瘤中有4个在IgJH（1个肿瘤）和C-λ（3个肿瘤）基因中显示重排。在这些肿瘤中观察到的基因重排模式不符合B细胞分化中描述的层次结构。这些结果表明，HD中的克隆群体不能明确归因于B细胞系。