运用聚合酶链反应检测霍奇金病中免疫球蛋白重链基因重排
Detection of immunoglobulin heavy chain gene rearrangements in Hodgkin's disease using PCR.
作者信息
Angel C A, Pringle J H, Primrose L, Lauder I
机构信息
Department of Pathology, University of Sheffield Medical School.
出版信息
J Clin Pathol. 1993 Oct;46(10):940-2. doi: 10.1136/jcp.46.10.940.
AIM
To detect clonal rearrangements of the immunoglobulin (Ig) heavy chain gene in Hodgkin's disease tissue using the polymerase chain reaction (PCR).
METHODS
DNA extracted from 36 samples of Hodgkin's disease was analysed using PCR and primers from conserved sequences in the variable (VH) and joining (JH) regions.
RESULTS
Clonal rearrangement was detected only in one case. Evidence of clonal immunoglobulin gene rearrangement had been detected previously in this case using conventional Southern blot analysis.
CONCLUSIONS
The sensitivity of the two techniques is equivalent and clonal Ig heavy chain gene rearrangements are rare in Hodgkin's disease.
目的
采用聚合酶链反应（PCR）检测霍奇金淋巴瘤组织中免疫球蛋白（Ig）重链基因的克隆性重排。
方法
运用PCR及来自可变区（VH）和连接区（JH）保守序列的引物，对36份霍奇金淋巴瘤样本提取的DNA进行分析。
结果
仅在1例样本中检测到克隆性重排。此前在该病例中运用传统Southern印迹分析已检测到克隆性免疫球蛋白基因重排的证据。
结论
两种技术的敏感性相当，且克隆性Ig重链基因重排在霍奇金淋巴瘤中罕见。
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