Clinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Marañón, Doctor Esquerdo, 46, 28007, Madrid, Spain.
Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain.
Eur J Clin Microbiol Infect Dis. 2024 Apr;43(4):797-803. doi: 10.1007/s10096-024-04764-4. Epub 2024 Feb 14.
Fourier-transform infrared (FTIR) spectroscopy has the potential to be used for bacterial typing and outbreak characterization. We evaluated FTIR for the characterization of an outbreak caused by Elizabethkingia miricola. During the 2020-2021 period, 26 isolates (23 clinical and 3 environmental) were collected and analyzed by FTIR (IR Biotyper) and core-genome MLST (cgMLST), in addition to antimicrobial susceptibility testing. FTIR spectroscopy and cgMLST showed that 22 of the isolates were related to the outbreak, including the environmental samples, with only one discordance between both methods. Then, FTIR is useful for E. miricola typing and can be easily implemented in the laboratory.
傅里叶变换红外（FTIR）光谱学有可能用于细菌分型和暴发特征描述。我们评估了 FTIR 用于鉴定由伊丽莎白菌引起的暴发。在 2020-2021 年期间，收集了 26 株分离株（23 株临床分离株和 3 株环境分离株），并通过 FTIR（IR Biotyper）和核心基因组 MLST（cgMLST）进行分析，此外还进行了抗菌药物敏感性测试。FTIR 光谱和 cgMLST 表明，22 株分离株与暴发有关，包括环境样本，两种方法只有一个不一致。因此，FTIR 可用于伊丽莎白菌的分型，并且可以在实验室中轻松实施。
