He Mingwang, Zhong Weiming, Dai Rongsi, Long Su, Zhou Ying, Zhang Tongping, Zhou Boyang, Tang Tao, Yang Linlin, Jiang Sifan, Xiao Wenbin, Fu YanJiao, Guo Jiajing, Gao Zhipeng
Fisheries College, Hunan Agricultural University, Changsha 410128, Hunan Province, China.
College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China.
Curr Res Microb Sci. 2025 Mar 23;8:100380. doi: 10.1016/j.crmicr.2025.100380. eCollection 2025.
is a gram-negative bacillus, a life-threatening pathogen in humans and animals. Linalool, a naturally occurring monoterpene alcohol found in plant volatile oils, exhibits highly effective antibacterial properties. This study investigated the antibacterial activity and mechanism of linalool against . Initially, linalool showed potent antibacterial activity against , with inhibition zone (ZOI), MIC, and MBC values of 36.41 ± 1.23 mm, 0.125 % (v/v, 1.0775 mg/mL), and 0.125 % (v/v, 1.0775 mg/mL), respectively. Secondly, it was observed by electron microscopy that linalool caused crumpling, depression, and size reduction of the cells. Linalool affected cell membrane integrity, causing membrane damage and rupture. Thirdly, transcriptome analysis suggested that linalool affected C5-branched-chain dicarboxylic acid metabolism and the biosynthesis of valine, leucine, and isoleucine, result in increased energy production to linalool stress. Linalool disrupted cell division and RNA function in , and the cells responded to linalool-induced oxidative damage by up-regulating the expression of and genes. Fourthly, metabolome analysis revealed an increase in metabolites related to the glycerophospholipid metabolic pathway and NADP content in , which may be a metabolic response to linalool stress. Taken together, these findings provide a theoretical basis for the antibacterial mechanism of linalool and suggest potential applications for preventing infections.
是一种革兰氏阴性杆菌，对人类和动物都是一种危及生命的病原体。芳樟醇是一种天然存在于植物挥发油中的单萜醇，具有高效抗菌特性。本研究调查了芳樟醇对……的抗菌活性及作用机制。最初，芳樟醇对……表现出强效抗菌活性，抑菌圈（ZOI）、最低抑菌浓度（MIC）和最低杀菌浓度（MBC）值分别为36.41±1.23毫米、0.125%（v/v，1.0775毫克/毫升）和0.125%（v/v，1.0775毫克/毫升）。其次，通过电子显微镜观察到芳樟醇导致细胞起皱、凹陷和尺寸减小。芳樟醇影响细胞膜完整性，导致膜损伤和破裂。第三，转录组分析表明芳樟醇影响C5支链二羧酸代谢以及缬氨酸、亮氨酸和异亮氨酸的生物合成，导致对芳樟醇胁迫的能量产生增加。芳樟醇扰乱了……中的细胞分裂和RNA功能，细胞通过上调……和……基因的表达来应对芳樟醇诱导的氧化损伤。第四，代谢组分析显示……中与甘油磷脂代谢途径相关的代谢物和烟酰胺腺嘌呤二核苷酸磷酸（NADP）含量增加，这可能是对芳樟醇胁迫的一种代谢反应。综上所述，这些发现为芳樟醇的抗菌机制提供了理论依据，并提示了预防……感染的潜在应用。
