Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Engineering Research Center for Fishery Major Diseases Control and Efficient Healthy Breeding Industrial Technology (GERCFT), Guangxi Academy of Sciences, Nanning, China.
China-ASEAN Modern Fishery Industry Technology Transfer Demonstration Center, Beibu Gulf Marine Industrial Research Institute, Guangxi Academy of Marine Sciences, Nanning, China.
Front Cell Infect Microbiol. 2023 Mar 14;13:1094050. doi: 10.3389/fcimb.2023.1094050. eCollection 2023.
The bacterium is a multispecies pathogen associated with meningitis-like disease that has been isolated from several amphibian species, including the bullfrog, but this is the first isolation in Guangxi. In the present study, the dominant bacteria were isolated from the brains of five bullfrogs with meningitis-like disease on a South China farm in Guangxi.
The NFEM01 isolate was identified by Gram staining; morphological observations; , and -based phylogenetic tree analysis; and physiochemical characterization and was subjected to drug sensitivity and artificial infection testing.
As a result of identification, the NFEM01 strain was found to be . An artificial infection experiment revealed that NFEM01 infected bullfrogs and could cause symptoms of typical meningitis-like disease. As a result of the bacterial drug sensitivity test, NFEM01 is highly sensitive to mequindox, rifampicin, enrofloxacin, nitrofural, and oxytetracycline and there was strong resistance to gentamicin, florfenicol, neomycin, penicillin, amoxicillin, doxycycline, and sulfamonomethoxine. This study provides a reference to further study the pathogenesis mechanism of -induced bullfrog meningitislike disease and its prevention and treatment.
是一种与脑膜炎样疾病相关的多物种病原体，已从几种蛙类物种中分离出来，包括牛蛙，但这是在广西的首次分离。本研究从广西华南农场的五只患有脑膜炎样疾病的牛蛙脑中分离出优势菌。
通过革兰氏染色、形态学观察、16S rRNA 基因序列分析和基于生理化学特征的系统发育树分析，对 NFEM01 分离株进行鉴定，并进行药敏试验和人工感染试验。
鉴定结果表明，NFEM01 菌株为 。人工感染实验表明，NFEM01 感染牛蛙并能引起典型脑膜炎样疾病的症状。细菌药敏试验结果表明，NFEM01 对美喹多辛、利福平、恩诺沙星、呋喃妥因和土霉素高度敏感，对庆大霉素、氟苯尼考、新霉素、青霉素、阿莫西林、强力霉素和磺胺甲恶唑有较强的耐药性。本研究为进一步研究 引起的牛蛙脑膜炎样疾病的发病机制及其防治提供了参考。
