The role of in tumor prognosis and immune infiltration: A Pan-cancer analysis.

PURPOSE

There is evidence that the Crystallin Alpha B gene is involved in the regulation of the tumor microenvironment and influences tumor prognosis in some cancers. However, the role of gene in prognosis and immunology in pan-cancer is still unclear.

METHODS

In this study, we analyzed the transcriptional profiles and survival data of cancer patients from The Cancer Genome Atlas (TCGA) database. gene and its relationships with pan-cancer were analyzed using R packages, TIMER2.0, GEPIA2, Sangerbox, UALCAN, cBioPortal, ESTIMATE algorithm, and STRING. Besides, real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) was utilized to detect expression in KIRC and a human KIRC cell line (Caki-1).

RESULTS

We found that expression was different in tumors and adjacent tumors in human cancers, affecting patients' prognosis in 15 cancer types. Additionally, expression significantly correlated with tumor microenvironment (TME), immune checkpoints (ICP), tumor mutational burden (TMB), and microsatellite instability (MSI) in human cancers. Besides, expression was positively associated with the immune infiltration of cancer-associated fibroblasts (CAFs) and endothelial cells in most human cancers. Based on enrichment analysis, the most prevalent gene mechanism in malignant tumors may be through anti-apoptotic activity. Moreover, some FDA-approved drugs were found to be associated with and might be potential cancer therapeutic candidates.

CONCLUSIONS

is a crucial component of the TME and influences immune cell infiltration, making it a promising biomarker to assess immune infiltration and prognosis in many malignancies.