Chen Huan, Zhu Weijian, Lu Jing, Fan Jinqing, Sun Luning, Feng Xiaoke, Liu Hao, Zhang Zhaohui, Wang Yongqing
Department of Acupuncture, The First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
Department of Pharmacy, The First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
PLoS One. 2016 Dec 8;11(12):e0166638. doi: 10.1371/journal.pone.0166638. eCollection 2016.
Interstitial cells of Cajal (ICCs) and nNOS play a crucial role in diabetic gastrointestinal dysmotility(DGD). Our previous study found that electro-acupuncture(EA) on ear point 'stomach' could repair the gastric dysrhythmias in rats induced by rectal distention(RD) after meal. However, little were known about the possible effect of auricular electro-acupuncture (AEA) on diabetic rats. Thus, we designed this study to investigate the effect of AEA on streptozotocin(STZ)-induced diabetic rats.
Forty male Sprague_Dawley (SD) rats were injected with STZ, at the end of 8th week after injection, animals were randomly divided into four groups and received 2 weeks-treatment(10 times) respectively: control group(CON,n = 10, no stimulation), sham auricular electro-acupuncture group(SEA,n = 10, low frequency EA on earlobes), auricular eletro-acupuncture group(AEA,n = 10, low frequency EA on ear point 'stomach'), and ST-36 group(ST-36,n = 10, low frequency EA on ST-36). Gastrointestinal (GI) motility was measured by GI transit rate. ICCs(c-kit+ expression) in antrum were analyzed by Immunohistochemistry and western blotting. NO level in blood serum were detected by Griess Reagent, and nNOSmRNA expression in antrum were determined by Real-time PCR.
GI transit rate and ICCs(c-kit+ expression) in antrum of AEA group have the tendency to increase compared with CON group, but had no statistics difference (P>0.05). nNOSmRNA expression in antrum of AEA group was dramatically increased compared with CON group (P = 0.037).
Low frequency EA on ear 'stomach' point could significantly up-regulate nNOS mRNA expression and ameliorate the ICCs networks partly in gastric antrum of STZ -induced diabetic rats, which may has benefits on regulating the GI motility.
Cajal间质细胞（ICCs）和神经元型一氧化氮合酶（nNOS）在糖尿病性胃肠动力障碍（DGD）中起关键作用。我们之前的研究发现，针刺耳穴“胃”可修复大鼠餐后直肠扩张（RD）诱导的胃节律紊乱。然而，关于耳针（AEA）对糖尿病大鼠的可能影响知之甚少。因此，我们设计了本研究来探讨AEA对链脲佐菌素（STZ）诱导的糖尿病大鼠的影响。
40只雄性Sprague-Dawley（SD）大鼠注射STZ，注射后第8周结束时，将动物随机分为四组，分别接受2周治疗（10次）：对照组（CON，n = 10，无刺激）、假耳针组（SEA，n = 10，耳垂低频电针）、耳针组（AEA，n = 10，耳穴“胃”低频电针）和足三里组（ST-36，n = 10，足三里低频电针）。通过胃肠传输率测量胃肠（GI）动力。通过免疫组织化学和蛋白质印迹分析胃窦中ICCs（c-kit +表达）。用Griess试剂检测血清中NO水平，用实时PCR测定胃窦中nNOSmRNA表达。
与CON组相比，AEA组胃窦的胃肠传输率和ICCs（c-kit +表达）有增加趋势，但无统计学差异（P>0.05）。与CON组相比，AEA组胃窦中nNOSmRNA表达显著增加（P = 0.037）。
耳穴“胃”低频电针可显著上调STZ诱导的糖尿病大鼠胃窦中nNOS mRNA表达并部分改善ICCs网络，这可能有利于调节胃肠动力。
