Cerchiara Teresa, Abruzzo Angela, Ñahui Palomino Rogers Alberto, Vitali Beatrice, De Rose Renata, Chidichimo Giuseppe, Ceseracciu Luca, Athanassiou Athanassia, Saladini Bruno, Dalena Francesco, Bigucci Federica, Luppi Barbara
Department of Pharmacy and Biotechnologies, University of Bologna, Via San Donato 19/2, 40127 Bologna, Italy.
Department of Pharmacy and Biotechnologies, University of Bologna, Via San Donato 19/2, 40127 Bologna, Italy.
Eur J Pharm Sci. 2017 Mar 1;99:105-112. doi: 10.1016/j.ejps.2016.11.028. Epub 2016 Dec 5.
In this work, we propose as new wound dressing, the Spanish Broom fibers impregnated with vancomycin (VM) loaded chitosan nanoparticles. Spanish Broom fibers were extracted by patented method DiCoDe and the morphological, physical and mechanical properties were investigated. Chitosan nanoparticles were prepared by ionic gelation using different weight ratios between chitosan (CH) and tripolyphosphate (TPP). Nanoparticles were characterized in terms of size, zeta potential, yield, encapsulation efficiency, stability and drug release. Finally, the antibacterial activity against Staphylococcus aureus as well as in vitro cytotoxicity on HaCaT cells were evaluated. The best formulation CH/TPP 4:1 was selected based on the encapsulation efficiency and yield. Spanish Broom fibers impregnated with loaded nanoparticles showed an increased antibacterial activity against S. aureus compared to the same fibers containing VM without nanoparticles. Moreover, these fibers were not toxic to HaCaT keratinocytes cells. In conclusion, Spanish Broom fibers impregnated with VM loaded CH/TPP nanoparticles would appear to be a promising candidate for wound dressing application.
在本研究中,我们提出将负载万古霉素(VM)的壳聚糖纳米颗粒浸渍的西班牙金雀花纤维作为新型伤口敷料。西班牙金雀花纤维通过专利方法DiCoDe提取,并对其形态、物理和力学性能进行了研究。通过离子凝胶法,使用壳聚糖(CH)和三聚磷酸钠(TPP)之间不同的重量比制备壳聚糖纳米颗粒。对纳米颗粒的尺寸、zeta电位、产率、包封率、稳定性和药物释放进行了表征。最后,评估了其对金黄色葡萄球菌的抗菌活性以及对HaCaT细胞的体外细胞毒性。基于包封率和产率选择了最佳配方CH/TPP 4:1。与含有VM但不含纳米颗粒的相同纤维相比,负载纳米颗粒的西班牙金雀花纤维对金黄色葡萄球菌表现出更高的抗菌活性。此外,这些纤维对HaCaT角质形成细胞无毒。总之,负载VM的CH/TPP纳米颗粒浸渍的西班牙金雀花纤维似乎是伤口敷料应用的一个有前途的候选材料。