Rossi Martina, Rossello Simone, Sallustio Valentina, Mandrone Manuela, Cerchiara Teresa, Chiocchio Ilaria, Chidichimo Giuseppe, Protti Michele, Mercolini Laura, Luppi Barbara, Bigucci Federica, Abruzzo Angela, Cappadone Concettina
Drug Delivery Research Laboratory, Department of Pharmacy and Biotechnology, Alma Mater Studiorum, University of Bologna, Via San Donato 19/2, 40127 Bologna, Italy.
Pharmaceutical Biochemistry Laboratory, Department of Pharmacy and Biotechnology, Alma Mater Studiorum, University of Bologna, Via San Donato 19/2, 40127 Bologna, Italy.
Pharmaceutics. 2025 May 9;17(5):632. doi: 10.3390/pharmaceutics17050632.
Over the last decade, the development of innovative wound products has continued to be a focus of intense research to meet the huge demand of patients. The aim of this work was to develop novel medicated Spanish broom wound dressings capable of releasing extract, recognized for its high antioxidant activity. extract was encapsulated in two different nanocarriers, namely glycethosomes and allanthosomes. The physico-chemical and functional characteristics of the obtained vesicles were described, including their size, particle size distribution, ζ potential, and encapsulation efficiency (EE). In addition, vesicles cytotoxicity and cell proliferation were evaluated on human fibroblasts. Furthermore, loaded vesicles were sunk into Spanish broom dressings, analyzed by confocal microscopy, and, finally, evaluated for their wound healing ability by scratch test. Both carriers are nanometric in size, with a good EE (>70%), and a negative ζ potential. Additionally, vesicles are biosafe, non-cytotoxic, and lead to complete closure of the scratch in about 30 h. The findings showed that the developed Spanish broom dressings have the potential to be an efficient and innovative wound care product for accelerating skin wounds.
在过去十年中，为满足患者的巨大需求，创新伤口护理产品的开发一直是深入研究的重点。这项工作的目的是开发新型含药染料木伤口敷料，其能够释放具有高抗氧化活性的染料木提取物。染料木提取物被包裹在两种不同的纳米载体中，即甘油脂质体和尿囊脂质体。描述了所得囊泡的物理化学和功能特性，包括其大小、粒径分布、ζ电位和包封率（EE）。此外，还评估了囊泡对人成纤维细胞的细胞毒性和细胞增殖情况。此外，将负载的囊泡嵌入染料木伤口敷料中，通过共聚焦显微镜进行分析，最后通过划痕试验评估其伤口愈合能力。两种载体尺寸均为纳米级，具有良好的包封率（>70%）和负ζ电位。此外，囊泡具有生物安全性，无细胞毒性，并能在约30小时内使划痕完全闭合。研究结果表明，所开发的染料木伤口敷料有潜力成为一种高效创新的伤口护理产品，用于加速皮肤伤口愈合。
