Guangdong Key Laboratory of Nanomedicine, CAS Key Lab for Health Informatics, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences , Shenzhen 518055, People's Republic of China.
University of Chinese Academy of Sciences , Beijing 100049, People's Republic of China.
ACS Nano. 2016 Nov 22;10(11):10049-10057. doi: 10.1021/acsnano.6b04695. Epub 2016 Nov 10.
An active cell membrane-camouflaged nanoparticle, owning to membrane antigens and membrane structure, can achieve special properties such as specific recognition, long blood circulation, and immune escaping. Herein, we reported a cancer cell membrane-cloaked nanoparticle system as a theranostic nanoplatform. The biomimetic nanoparticles (indocyanine green (ICG)-loaded and cancer cell membrane-coated nanoparticles, ICNPs) exhibit a core-shell nanostructure consisting of an ICG-polymeric core and cancer cell membrane shell. ICNPs demonstrated specific homologous targeting to cancer cells with good monodispersity, preferable photothermal response, and excellent fluorescence/photoacoustic (FL/PA) imaging properties. Benefited from the functionalization of the homologous binding adhesion molecules from cancer cell membranes, ICNPs significantly promoted cell endocytosis and homologous-targeting tumor accumulation in vivo. Moreover, ICNPs were also good at disguising as cells to decrease interception by the liver and kidney. Through near-infrared (NIR)-FL/PA dual-modal imaging, ICNPs could realize real-time monitored in vivo dynamic distribution with high spatial resolution and deep penetration. Under NIR laser irradiation, ICNPs exhibited highly efficient photothermal therapy to eradicate xenografted tumor. The robust ICNPs with homologous properties of cancer cell membranes can serve as a bionic nanoplatform for cancer-targeted imaging and phototherapy.
一种主动细胞膜伪装纳米粒子，由于具有膜抗原和膜结构，能够实现特异性识别、长血液循环和免疫逃避等特殊性质。在此，我们报道了一种癌细胞膜包裹的纳米粒子系统作为一种治疗诊断两用的纳米平台。仿生纳米粒子（载有吲哚菁绿（ICG）和癌细胞膜包裹的纳米粒子，简称 ICNPs）具有核壳结构，由 ICG-聚合物核和癌细胞膜壳组成。ICNPs 表现出对癌细胞的特异性同源靶向，具有良好的单分散性、优异的光热响应和出色的荧光/光声（FL/PA）成像性能。得益于癌细胞膜上同源结合粘附分子的功能化，ICNPs 显著促进了细胞内吞作用和同源靶向肿瘤在体内的积累。此外，ICNPs 还善于伪装成细胞，减少被肝脏和肾脏拦截。通过近红外（NIR）FL/PA 双模态成像，ICNPs 可以实现具有高空间分辨率和深穿透性的体内动态分布的实时监测。在近红外激光照射下，ICNPs 表现出高效的光热治疗作用，能够根除异种移植肿瘤。具有癌细胞膜同源特性的坚固 ICNPs 可以作为一种仿生纳米平台，用于癌症靶向成像和光疗。
