Kırmızıbekmez Hasan, Tiftik Kubilay, Kúsz Norbert, Orban-Gyapai Orsolya, Zomborszki Zoltán Péter, Hohmann Judit
Department of Pharmacognosy, Faculty of Pharmacy, Yeditepe University, TR-34755, Kayışdağı, İstanbul, Turkey.
Faculty of Pharmacy, Yeditepe University, TR-34755, Kayışdağı, İstanbul, Turkey.
Chem Biodivers. 2017 Mar;14(3). doi: 10.1002/cbdv.201600288. Epub 2017 Feb 18.
Three new iridoid glycosides, named involucratosides A - C (1 - 3), were isolated from the H O subextract of crude MeOH extract prepared from the aerial parts of Asperula involucrata along with a known iridoid glycoside (adoxoside), three flavone glycosides (apigenin 7-O-β-glucopyranoside, luteolin 7-O-β-glucopyranoside, apigenin 7-O-rutinoside) as well as two phenolic acid derivatives (chlorogenic acid and ferulic acid 4-O-β-glucopyranoside). Their chemical structures were established by UV, IR, 1D- ( H, C and JMOD) and 2D- (COSY, HSQC, HMBC and NOESY) NMR experiments and HR-ESI-MS. In addition, the crude extract, subextracts and isolates were evaluated for their xanthine oxidase inhibitory and antioxidant activities in in vitro tests. This is the first report on the chemical composition and bioactivities of A. involucrata.
从总状猪殃殃地上部分制备的甲醇粗提取物的水提取物中分离得到了三种新的环烯醚萜苷，命名为总状猪殃殃苷A - C（1 - 3），同时还得到了一种已知的环烯醚萜苷（五福花苷）、三种黄酮苷（芹菜素7 - O - β - 葡萄糖吡喃糖苷、木犀草素7 - O - β - 葡萄糖吡喃糖苷、芹菜素7 - O - 芸香糖苷）以及两种酚酸衍生物（绿原酸和阿魏酸4 - O - β - 葡萄糖吡喃糖苷）。通过紫外光谱（UV）、红外光谱（IR）、一维核磁共振（¹H、¹³C和JMOD）以及二维核磁共振（COSY、HSQC、HMBC和NOESY）实验和高分辨电喷雾电离质谱（HR - ESI - MS）确定了它们的化学结构。此外，在体外试验中对粗提取物、提取物和分离物的黄嘌呤氧化酶抑制活性和抗氧化活性进行了评估。这是关于总状猪殃殃化学成分和生物活性的首次报道。
