Ghazal Aghiad, Gontsarik Mark, Kutter Jörg P, Lafleur Josiane P, Ahmadvand Davoud, Labrador Ana, Salentinig Stefan, Yaghmur Anan
Niels Bohr Institute, University of Copenhagen , Universitetsparken 5, DK-2100 Copenhagen Ø, Denmark.
Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen , Universitetsparken 2, DK-2100 Copenhagen Ø, Denmark.
J Phys Chem Lett. 2017 Jan 5;8(1):73-79. doi: 10.1021/acs.jpclett.6b02468. Epub 2016 Dec 12.
A microfluidic platform combined with synchrotron small-angle X-ray scattering (SAXS) was used for monitoring the continuous production of multilamellar vesicles (MLVs). Their production was fast and started to evolve within less than 0.43 s of contact between the lipids and the aqueous phase. To obtain nanoparticles with a narrow size distribution, it was important to use a modified hydrodynamic flow focusing (HFF) microfluidic device with narrower microchannels than those normally used for SAXS experiments. Monodispersed MLVs as small as 160 nm in size, with a polydispersity index (PDI) of approximately 0.15 were achieved. The nanoparticles produced were smaller and had a narrower size distribution than those obtained via conventional bulk mixing methods. This microfluidic platform therefore has a great potential for the continuous production of monodispersed NPs.
一个结合了同步加速器小角X射线散射（SAXS）的微流控平台被用于监测多层囊泡（MLV）的连续生产。它们的生产速度很快，在脂质与水相接触不到0.43秒内就开始形成。为了获得具有窄尺寸分布的纳米颗粒，使用一种改进的流体动力学流动聚焦（HFF）微流控装置很重要，该装置的微通道比通常用于SAXS实验的微通道更窄。实现了尺寸小至160nm、多分散指数（PDI）约为0.15的单分散MLV。所生产的纳米颗粒比通过传统批量混合方法获得的纳米颗粒更小且尺寸分布更窄。因此，这个微流控平台在连续生产单分散纳米颗粒方面具有巨大潜力。
