Huang Ching-Yuang, Huang Tsui-Hsien, Kao Chia-Tze, Wu Yuan-Haw, Chen Wan-Chen, Shie Ming-You
Department of Stomatology, Chang Bing Show Chwan Memorial Hospital, Changhua, Taiwan; Division of Family Dentistry, Chang Bing Show Chwan Memorial Hospital, Changhua, Taiwan.
School of Dentistry, Chung Shan Medical University, Taichung, Taiwan; Department of Stomatology, Chung Shan Medical University Hospital, Taichung, Taiwan.
J Endod. 2017 Jan;43(1):69-76. doi: 10.1016/j.joen.2016.09.012. Epub 2016 Dec 6.
Calcium silicate (CS) -based materials play an important role in the development of endodontic materials that induce bone/cementum tissue regeneration and inhibit bacterial viability. The aim of this study was to prepare novel mesoporous CS (MesoCS) nanoparticles that have osteogenic, drug delivery, and antibacterial characteristics for endodontic materials and also have an excellent ability to develop apatite mineralization.
The MesoCS nanoparticles were prepared using sol-gel methods. In addition, the mesoporous structure, specific surface area, pore volume, and morphology of the MesoCS nanoparticles were analyzed. The apatite mineralization ability, in vitro odontogenic differentiation, drug delivery, and antibacterial properties of the MesoCS nanoparticles were further investigated.
The results indicate that the 200-nm-sized MesoCS nanoparticles synthesized using a facile template method exhibited a high specific surface area and pore volume with internal mesopores (average pore size = 3.05 nm). Furthermore, the MesoCS nanoparticles can be used as drug carriers to maintain sustained release of gentamicin and fibroblast growth factor-2 (FGF-2). The MesoCS-loaded FGF-2 might stimulate more odontogenic-related protein than CS because of the FGF-2 release.
Based on this work, it can be inferred that MesoCS nanoparticles are potentially useful endodontic materials for biocompatible and osteogenic dental pulp tissue regenerative materials.
硅酸钙（CS）基材料在诱导骨/牙骨质组织再生并抑制细菌活力的牙髓材料开发中发挥着重要作用。本研究的目的是制备新型介孔CS（MesoCS）纳米颗粒，其具有用于牙髓材料的成骨、药物递送和抗菌特性，并且还具有优异的磷灰石矿化能力。
采用溶胶-凝胶法制备MesoCS纳米颗粒。此外，分析了MesoCS纳米颗粒的介孔结构、比表面积、孔体积和形态。进一步研究了MesoCS纳米颗粒的磷灰石矿化能力、体外牙源性分化、药物递送和抗菌性能。
结果表明，使用简便模板法合成的尺寸为200nm的MesoCS纳米颗粒表现出高比表面积和孔体积以及内部介孔（平均孔径 = 3.05nm）。此外，MesoCS纳米颗粒可用作药物载体，以维持庆大霉素和成纤维细胞生长因子-2（FGF-2）的持续释放。由于FGF-2的释放，负载MesoCS的FGF-2可能比CS刺激更多与牙源性相关的蛋白质。
基于这项工作，可以推断MesoCS纳米颗粒是用于生物相容性和成骨性牙髓组织再生材料的潜在有用的牙髓材料。
