Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
Biosens Bioelectron. 2017 Apr 15;90:343-348. doi: 10.1016/j.bios.2016.11.030. Epub 2016 Nov 12.
In this work, we demonstrate a novel method based on inductively coupled plasma mass spectrometry (ICP-MS) detection with gold nanoparticles (Au NPs) and quantum dots (QDs) labeling for the simultaneous counting of two circulating tumor cell lines (MCF-7 and HepG2 cells) in human blood. MCF-7 and HepG2 cells were captured by magnetic beads coupled with anti-EpCAM and then specifically labeled by CdSe QDs-anti-ASGPR and Au NPs-anti-MUC1, respectively, which were used as signal probes for ICP-MS measurement. Under the optimal experimental conditions, the limits of detection of 50 MCF-7, 89 HepG2 cells and the linear ranges of 200-40000 MCF-7, 300-30000 HepG2 cells were obtained, and the relative standard deviations for seven replicate detections of 800 MCF-7 and HepG2 cells were 4.6% and 5.7%, respectively. This method has the advantages of high sensitivity, low sample consumption, wide linear range and can be extended to the simultaneous detection of multiple CTC lines in human peripheral blood.
在这项工作中，我们展示了一种基于电感耦合等离子体质谱（ICP-MS）检测的新方法，该方法结合了金纳米粒子（Au NPs）和量子点（QDs）标记，用于同时计数人血液中的两种循环肿瘤细胞系（MCF-7 和 HepG2 细胞）。MCF-7 和 HepG2 细胞被与抗 EpCAM 偶联的磁性珠捕获，然后分别被 CdSe QDs-抗 ASGPR 和 Au NPs-抗 MUC1 特异性标记，它们被用作 ICP-MS 测量的信号探针。在最佳实验条件下，获得了 50 MCF-7、89 HepG2 细胞的检测限和 200-40000 MCF-7、300-30000 HepG2 细胞的线性范围，7 次重复检测 800 MCF-7 和 HepG2 细胞的相对标准偏差分别为 4.6%和 5.7%。该方法具有灵敏度高、样品消耗少、线性范围宽的优点，可扩展用于同时检测人外周血中的多种 CTC 系。
