Hwang D F, Wang W C, Chung H M, Jeng S S
Taiwan Yi Xue Hui Za Zhi. 1989 Mar;88(3):289-91.
Attempt was made to elucidate the responsible toxin in fish which recently caused food poisoning in Ilan County, Taiwan. The causative fish was assayed for anatomical distribution of toxicity (as tetrodotoxin). The roe showed the highest toxicity (1200 mouse units per gram, abbreviated as MU/g), followed by the dermis (150 MU/g) and muscle (120 MU/g). The toxins were partially purified from the methanolic extracts of the fish muscle and roe by ultrafiltration and Bio-Gel P-2 column chromatography. Thin-layer chromatography, cellulose acetate membrane electrophoresis and high performance liquid chromatography analysis demonstrated that the toxins consisted of tetrodotoxin and anhydrotetrodotoxin. It was concluded that the causative agent of the above food poisoning was tetrodotoxin.
为阐明近期在台湾宜兰县引发食物中毒的鱼类中的致病毒素，对致病鱼类进行了毒性（以河豚毒素计）的解剖分布测定。鱼卵的毒性最高（每克1200小鼠单位，缩写为MU/g），其次是真皮（150 MU/g）和肌肉（120 MU/g）。通过超滤和Bio-Gel P-2柱色谱法从鱼肌肉和鱼卵的甲醇提取物中对毒素进行了部分纯化。薄层色谱、醋酸纤维素膜电泳和高效液相色谱分析表明，毒素由河豚毒素和脱水河豚毒素组成。得出结论，上述食物中毒的致病因子为河豚毒素。
