San-Galli F, Vrignaud P, Robert J, Coindre J M, Cohadon F
Laboratoire de Neurochirurgie expérimentale et Neurobiologie, Université de Bordeaux II, France.
J Neurooncol. 1989 Sep;7(3):299-304. doi: 10.1007/BF00172924.
Establishing in vivo glioblastoma models from cell lines requires a very strict methodology, in order to obtain reproducible tumors presenting all the characteristics of human spontaneous glioblastomas. In this respect, we have developed a model of glioblastoma in Wistar rats by stereotaxic intracerebral transplantation of a 10 microliters suspension of 6 x 10(6) C6 cells grown in vitro. The tumor take was very high in these conditions, only 1 rat over 30 had no tumor. The median survival time varied from 14 to 20 days. The growth curve of the tumor has revealed an exponential growth up to the 10th day after transplantation with a doubling time of 36 h. Histological examination of the tumors has shown several characteristic features of spontaneous glioblastomas, such as neovasculature, parenchymal invasion, nuclear pleiomorphism, and presence of hemorrhagic and necrotic areas. This C6 model is closer to the usual histological characteristics of spontaneous glioblastomas when using the Wistar rather than other strain, and it should be used in that way for preclinical therapeutic evaluation of new drugs or drug combinations in glioblastoma.
从细胞系建立体内胶质母细胞瘤模型需要非常严格的方法，以便获得具有人类自发性胶质母细胞瘤所有特征的可重复性肿瘤。在这方面，我们通过立体定向脑内移植10微升体外培养的6×10⁶个C6细胞悬液，在Wistar大鼠中建立了胶质母细胞瘤模型。在这些条件下肿瘤形成率非常高，30只大鼠中只有1只没有肿瘤。中位生存时间为14至20天。肿瘤生长曲线显示移植后第10天前呈指数生长，倍增时间为36小时。肿瘤的组织学检查显示出一些自发性胶质母细胞瘤的特征，如新生血管形成、实质浸润、核多形性以及出血和坏死区域的存在。当使用Wistar大鼠而非其他品系时，这种C6模型更接近自发性胶质母细胞瘤的常见组织学特征，应以这种方式用于胶质母细胞瘤新药或药物组合的临床前治疗评估。
