Perry H M, Erlanger M W, Gustafsson T O, Perry E F
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110.
J Toxicol Environ Health. 1989;28(2):151-9. doi: 10.1080/15287398909531336.
The aim of this experiment was to test whether a chelating agent, D-myo-inositol-1,2,6-trisphosphate (PP56), could reverse cadmium-induced hypertension. Four groups of weanling female Long-Evans rats received ad libitum a rye-based, metal-poor diet and deionized water fortified with essential metals for 15 mo from the time of weaning. A control group received neither cadmium nor chelating agent. A second group had 0.1 ppm cadmium added to their water from weaning through mo 5. A third group had 60 ppm PP56 added to their water for mo 6-10. The fourth group had 0.1 ppm cadmium added to their water from weaning through mo 5 and 60 ppm PP56 from mo 6-10. All groups were followed without either cadmium or PP56 for mo 11-15. At approximately monthly intervals, systolic pressure was measured by the indirect tail cuff method in unanesthetized rats. Chronic cadmium feeding induced the expected hypertension, with an average increase in systolic pressure of about 15 mm Hg; the pressor effect persisted with little change for the 10 mo after cadmium was withdrawn. PP56 completely reversed the cadmium-induced hypertension, and the inhibition persisted for 5 mo after PP56 was withdrawn. PP56 by itself had no demonstrable depressor effect.
本实验的目的是测试一种螯合剂，D-肌醇-1,2,6-三磷酸（PP56）是否能逆转镉诱导的高血压。四组断奶雌性Long-Evans大鼠从断奶时起自由摄取以黑麦为基础的低金属饮食和添加了必需金属的去离子水，持续15个月。对照组既未接受镉也未接受螯合剂。第二组从断奶到第5个月在饮水中添加0.1 ppm镉。第三组在第6至10个月在饮水中添加60 ppm PP56。第四组从断奶到第5个月在饮水中添加0.1 ppm镉，从第6至10个月添加60 ppm PP56。在第11至15个月，所有组均不再接触镉或PP56。大约每隔一个月，用间接尾套法在未麻醉的大鼠中测量收缩压。长期喂食镉会导致预期的高血压，收缩压平均升高约15 mmHg；在停止喂食镉后的10个月内，升压作用几乎没有变化且持续存在。PP56完全逆转了镉诱导的高血压，并且在停用PP56后，这种抑制作用持续了5个月。PP56本身没有明显的降压作用。
