Beuker Janina, Barth Theresa, Steier Anke, Wittgens Andreas, Rosenau Frank, Henkel Marius, Hausmann Rudolf
Department of Bioprocess Engineering (150k), Institute of Food Science and Biotechnology, University of Hohenheim, Fruwirthstr. 12, 70599, Stuttgart, Germany.
Evonik Industries, Evonik Technology and Infrastructure GmbH, Rodenbacher Chaussee 4, 63457, Hanau-Wolfgang, Germany.
AMB Express. 2016 Dec;6(1):124. doi: 10.1186/s13568-016-0298-5. Epub 2016 Dec 12.
Heterologous mono-rhamnolipid production by Pseudomonas putida KT2440 pSynPro8oT_rhlAB using glucose as the single carbon source was characterized in fed-batch bioreactor cultivations. For the described experiments, a defined mineral salt medium was used, and a two phase glucose feeding profile was applied, which yielded a final rhamnolipid concentration of 14.9 g/L. Applying the feeding profile, glucose stayed almost constant until 28 h of cultivation and decreased afterwards to limiting levels. Until the end of cultivation 253.0 ± 0.1 g glucose was added to the bioreactor of which a total of 252.0 ± 0.6 g glucose was metabolized. By modeling the fed-batch bioreactor cultivations the time courses of generated biomass, rhamnolipid and consumed glucose were described. The model was furthermore used to derive key process parameters from the collected data. The obtained values for the specific product formation rates (q) reached 18 mg/(g h) and yield coefficients (Y) 10 mg/g respectively.
在补料分批生物反应器培养中，对以葡萄糖作为唯一碳源的恶臭假单胞菌KT2440 pSynPro8oT_rhlAB产生异源单鼠李糖脂的情况进行了表征。对于所描述的实验，使用了一种确定的无机盐培养基，并采用了两阶段葡萄糖补料曲线，最终鼠李糖脂浓度达到14.9 g/L。采用该补料曲线时，葡萄糖在培养28小时前几乎保持恒定，之后降至限制水平。到培养结束时，向生物反应器中添加了253.0±0.1 g葡萄糖，其中总共252.0±0.6 g葡萄糖被代谢。通过对补料分批生物反应器培养进行建模，描述了生成的生物量、鼠李糖脂和消耗的葡萄糖的时间进程。该模型还用于从收集的数据中推导关键工艺参数。得到的特定产物形成速率（q）值分别达到18 mg/(g h)，产率系数（Y）为10 mg/g。
