Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei Key Laboratory of Polymer Materials, School of Materials Science & Engineering, Hubei University , Wuhan 430062, China.
Division of Spine Surgery, Department of Orthopaedics & Traumatology, Li Ka Shing Faculty of Medicine, The University of Hong Kong , Pokfulam, Hong Kong, China.
ACS Appl Mater Interfaces. 2016 Dec 14;8(49):33972-33981. doi: 10.1021/acsami.6b09457. Epub 2016 Nov 30.
A hybrid coating composed of hydroxyapatite (HA), Ag nanoparticles (NPs), and chitosan (CS) was successfully prepared on a Ti substrate by a layer-by-layer assembly process. A polydopamine-assisted (PDA-assisted) coating showed a good bond with HA. Ag NPs were uniformly distributed into the hybrid coating through a solution method and ultraviolet light reduction. A CS nanofilm was deposited via spin-coating to control the release of Ag from the hybrid coating. The results disclosed that the 3-layer CS coating could efficiently control the release of Ag from the hybrid coating via the Fickian diffusion mechanism and that the PDA/HA/Ag/CS-1 coating exhibited antibacterial ratios of 63.0% and 51.8% against E. coli and S. aureus, respectively. Furthermore, the normal structure of E. coli was obviously destroyed by two types of Ag doped coatings. The cell viability assay showed that CS effectively reduced the cytotoxicity of the hybrid coating after a 7 day incubation. The hybrid coating presented high ALP activities at days 3 and 14. The results reveal that hybrid coatings can endow Ti implants with good antibacterial capability as well as cell viability and osteogenic activity.
通过层层组装工艺，成功地在 Ti 基底上制备了由羟基磷灰石 (HA)、Ag 纳米粒子 (NPs) 和壳聚糖 (CS) 组成的杂化涂层。通过多巴胺辅助 (PDA 辅助) 涂层显示出与 HA 的良好结合。通过溶液法和紫外光还原将 Ag NPs 均匀地分布到杂化涂层中。通过旋涂沉积 CS 纳米薄膜来控制 Ag 从杂化涂层中的释放。结果表明，3 层 CS 涂层可以通过菲克扩散机制有效地控制 Ag 从杂化涂层中的释放，并且 PDA/HA/Ag/CS-1 涂层对大肠杆菌和金黄色葡萄球菌的抑菌率分别为 63.0%和 51.8%。此外，两种类型的 Ag 掺杂涂层明显破坏了大肠杆菌的正常结构。细胞活力测定表明，CS 有效地降低了杂化涂层在 7 天孵育后的细胞毒性。在第 3 天和第 14 天，杂化涂层呈现出高的碱性磷酸酶活性。结果表明，杂化涂层可以赋予 Ti 植入物良好的抗菌能力以及细胞活力和成骨活性。
