Padakandla Shalem Raj, Chae Jong-Chan
Division of Biotechnology, Chonbuk National University, Iksan 54596, Republic of Korea.
Advanced Institute of Environmental and Bioscience, Chonbuk National University, Iksan 54596, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Apr;67(4):1018-1023. doi: 10.1099/ijsem.0.001734. Epub 2017 May 5.
A Gram-reaction-negative, rod-shaped, facultatively anaerobic, motile bacterium, designated strain AR1T, was isolated from a freshwater stream in Jeonju, South Korea. Strain AR1T showed highest 16S rRNA gene sequence similarity (96.83 %) and also formed a separate clade with Aeromonas sharmana GPTSA-6T in the phylogenetic tree reconstructed among the members of the family Aeromonadaceae. Major cellular fatty acids are summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and C16: 0. Diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol are the predominant polar lipids. The genomic DNA G+C content was found to be 54.7 mol%. However, earlier studies on 16S rRNA gene, gyrB, rpoD and universal target region of cpn60 sequences of the members of the genus Aeromonas recommended the transfer of Aeromonas sharmana to a new genus. Hence, based on the comparative polyphasic data obtained during the present study and also on the previous recommendations, it is proposed that Aeromonas sharmana be transferred to a novel genus as Pseudaeromonas sharmana gen. nov., comb. nov. with strain GPTSA-6T (=DSM 17445T=MTCC 7090T=CIP 109378T=CCUG 54939T) as the type strain of the type species of the genus. Also, it is proposed that strain AR1T be designated as a representative of a novel species of this new genus, namely Pseudaeromonas pectinilytica sp. nov. The type strain is AR1T (=KCTC 42754T=JCM 31503T).
从韩国全州的一条淡水溪流中分离出一株革兰氏阴性、杆状、兼性厌氧、运动的细菌，命名为AR1T菌株。AR1T菌株在气单胞菌科成员重建的系统发育树中显示出最高的16S rRNA基因序列相似性（96.83%），并且还与沙氏气单胞菌GPTSA-6T形成了一个单独的分支。主要细胞脂肪酸为特征性总和3（C16 : 1ω7c/C16 : 1ω6c）和C16: 0。双磷脂酰甘油、磷脂酰乙醇胺和磷脂酰甘油是主要的极性脂质。基因组DNA G+C含量为54.7 mol%。然而，早期对气单胞菌属成员的16S rRNA基因、gyrB、rpoD和cpn60序列通用靶区的研究建议将沙氏气单胞菌转移到一个新属。因此，基于本研究期间获得的比较多相数据以及先前的建议，提议将沙氏气单胞菌转移到一个新属，即果胶假气单胞菌属新属，新组合。菌株GPTSA-6T（=DSM 17445T=MTCC 7090T=CIP 109378T=CCUG 54939T）作为该属模式种的模式菌株。此外，提议将AR1T菌株指定为这个新属的一个新物种的代表，即果胶假气单胞菌新种。模式菌株为AR1T（=KCTC 42754T=JCM 31503T）。
