Akter Sumi, Shin Mann Kyoon
Department of Biological Science, University of Ulsan, Ulsan 44610, Republic of Korea.
Department of Botany, Jagannath University, Dhaka 1100, Bangladesh.
Int J Syst Evol Microbiol. 2018 Jun;68(6):1845-1850. doi: 10.1099/ijsem.0.002746.
A Gram-stain-negative, oxidase-negative and catalase-negative, motile, rod-shaped bacterial strain, designated PCS8, hosted by the ciliate was investigated by using a polyphasic approach. Strain PCS8 was observed to be able to grow at 12-44 °C (optimum, 36-37 °C), at pH 6.0-10.0 (optimum, 7.0) and in the presence of 0-3 % NaCl (optimum, 1-2 %). It could hydrolyse starch and aesculin and produce acid from d-sorbitol, -inositol, glycerol and l-rhamnose. The sequence similarity of the new isolate was 96.9 % with respect to and 96.3 % with respect to . Phylogenetically, strain PCS8 falls within the cluster comprising the species. The predominant cellular fatty acids of strain PCS8 were C (35.8 %), summed feature 3 (Cω7 and/or Cω6; 35.1 %) and summed feature 8 (Cω7 and/or Cω6; 10.8 %). This novel strain also contained various fatty acids that are not detected in other members of the genus , such as C 3-OH, Cω9 and summed feature 5 (Cω6,9 and/or C ante). Strain PCS8 contained ubiquinone-8 as the sole respiratory quinone and phosphatidylethanolamine and phosphatidylglycerol as major polar lipids. The G+C content of the genomic DNA of the type strain was 66.5 mol%. Based on the distinct phenotypic, phylogenetic, chemotaxonomic and G+C content results, strain PCS8 represents a currently undescribed species within the genus in the family , for which we suggest the name sp. nov. with the type strain PCS8 (=KCTC 62038=JCM 32226). An emended description of the genus is also provided.
采用多相分类法对一株由纤毛虫携带的革兰氏阴性、氧化酶阴性、过氧化氢酶阴性、具运动性的杆状细菌菌株PCS8进行了研究。观察到菌株PCS8能够在12 - 44 °C（最适温度为36 - 37 °C）、pH 6.0 - 10.0（最适pH为7.0）以及0 - 3 % NaCl（最适盐浓度为1 - 2 %）的条件下生长。它能够水解淀粉和七叶苷，并从d - 山梨醇、肌醇、甘油和L - 鼠李糖产酸。新分离菌株与[具体菌株1]的序列相似性为96.9 %，与[具体菌株2]的序列相似性为96.3 %。在系统发育上，菌株PCS8属于包含[具体属名]物种的簇。菌株PCS8的主要细胞脂肪酸为C（35.8 %）、特征性总和3（Cω7和/或Cω6；35.1 %）和特征性总和8（Cω7和/或Cω6；10.8 %）。该新菌株还含有在该属其他成员中未检测到的各种脂肪酸，如C 3 - OH、Cω9和特征性总和5（Cω6,9和/或C ante）。菌株PCS8含有泛醌 - 8作为唯一的呼吸醌，磷脂酰乙醇胺和磷脂酰甘油作为主要极性脂质。模式菌株基因组DNA的G + C含量为66.5 mol%。基于独特的表型、系统发育、化学分类和G + C含量结果，菌株PCS8代表了[具体科名]科[具体属名]属内一个目前未描述的物种，我们建议将其命名为[具体种名] sp. nov.，模式菌株为PCS8（=KCTC 62038 = JCM 32226）。同时还提供了[具体属名]属的修订描述。
