Alvarez Rojas Cristian A, Ebi Dennis, Paredes Rodolfo, Acosta-Jamett Gerardo, Urriola Nicole, Roa Juan Carlos, Manterola Carlos, Cortes Sandra, Romig Thomas, Scheerlinck Jean-Pierre, Lightowlers Marshall W
Faculty of Veterinary and Agricultural Sciences, Centre for Animal Biotechnology, The University of Melbourne, Parkville, Victoria 3010, Australia.
Parasitology Unit, University of Hohenheim, Emil-Wolff-Str. 34, 70599 Stuttgart, Germany.
Parasitol Int. 2017 Apr;66(2):112-115. doi: 10.1016/j.parint.2016.12.001. Epub 2016 Dec 11.
Echinococcus granulosus sensu stricto is the major cause of cystic echinococcosis in most human and animal cases in the world and the most widespread species within the E. granulosus sensu lato complex. E. granulosus s.s. remains endemic in South America together with other species of the Echinococcus genus, especially in some areas in Argentina, Brazil, Chile and Peru. Except for a single human case caused by E. canadensis (G6) described in the literature, only E. granulosus s.s. has been found in the Chilean territory. In the current study 1609bp of the cox1 gene from 69 Chilean isolates of E. granulosus s.s. from humans and animals were analysed. In total, 26 cox1 haplotypes were found, including the widespread haplotype EG01 (22 isolates) and also EGp1 (5), EgRUS7 (1), EgAus02 (1) and EgAus03 (2). Twenty-one different haplotype not previously described were identified from 38 Chilean isolates designated EgCL1-EgCL21. Previous work had described low variability of E. granulosus s.s. in South America, based on isolates from Peru. Results obtained in this work challenge the previously described idea of the low diversity of the parasite in South America, and warrant future investigation on the origin and spread of the parasite in the continent after the Spanish arrival.
细粒棘球绦虫狭义种是世界上大多数人类和动物囊型棘球蚴病的主要病因，也是细粒棘球绦虫复合种中分布最广的物种。细粒棘球绦虫狭义种与棘球属的其他物种一起在南美洲仍然呈地方性流行，特别是在阿根廷、巴西、智利和秘鲁的一些地区。除了文献中描述的由加拿大棘球绦虫（G6）引起的一例人类病例外，在智利境内仅发现了细粒棘球绦虫狭义种。在本研究中，对来自智利人和动物的69株细粒棘球绦虫狭义种分离株的细胞色素氧化酶亚基1（cox1）基因的1609bp进行了分析。总共发现了26种cox1单倍型，包括广泛分布的单倍型EG01（22株分离株）以及EGp1（5株）、EgRUS7（1株）、EgAus02（1株）和EgAus03（2株）。从38株命名为EgCL1 - EgCL21的智利分离株中鉴定出21种先前未描述的不同单倍型。此前的研究基于来自秘鲁的分离株描述了南美洲细粒棘球绦虫狭义种的低变异性。本研究获得的结果对先前描述的该寄生虫在南美洲低多样性的观点提出了挑战，并且有必要对西班牙人到来后该寄生虫在该大陆的起源和传播进行进一步研究。
