Avila Héctor G, Santos Guilherme B, Cucher Marcela A, Macchiaroli Natalia, Pérez Matías G, Baldi Germán, Jensen Oscar, Pérez Verónica, López Raúl, Negro Perla, Scialfa Exequiel, Zaha Arnaldo, Ferreira Henrique B, Rosenzvit Mara, Kamenetzky Laura
Instituto de Investigaciones en Microbiología y Parasitología Médica, IMPAM (UBA-CONICET), Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, piso 13 (1121), Ciudad Autónoma de Buenos Aires, Argentina; Centro de Investigaciones en Zoonosis, Chacra N°18, Sarmiento, Chubut, Argentina.
Programa de Pós-Graduação em Biologia Celular e Molecular, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Caixa Postal 15005, CEP 91501-970, Porto Alegre, RS, Brazil.
Parasitol Int. 2017 Jun;66(3):250-257. doi: 10.1016/j.parint.2017.02.001. Epub 2017 Feb 11.
The aim of this work was to determine Echinococcus granulosus sensu lato species and genotypes in intermediate and definitive hosts and in human isolates from endemic regions of Argentina and Brazil including those where no molecular data is available by a combination of classical and alternative molecular tools. A total of 227 samples were isolated from humans, natural intermediate and definitive hosts. Amplification of cytochrome c oxidase subunit I gene fragment was performed and a combination of AluI digestion assay, High Resolution Melting analysis (HRM) assay and DNA sequencing was implemented for Echinococcus species/genotype determination. E. granulosus sensu stricto (G1) was found in sheep (n=35), cattle (n=67) and dogs (n=5); E. ortleppi (G5) in humans (n=3) and cattle (n=108); E. canadensis (G6) in humans (n=2) and E. canadensis (G7) in pigs (n=7). We reported for the first time the presence of E. ortleppi (G5) and E. canadensis (G6) in humans from San Juan and Catamarca Argentinean provinces and E. canadensis (G7) in pigs from Cordoba Argentinean province. In this work, we widened molecular epidemiology studies of E. granulosus s. l. in South America by analyzing several isolates from definitive and intermediate hosts, including humans from endemic regions were such information was scarce or unavailable. The presence of different species/genotypes in the same region and host species reinforce the need of rapid and specific techniques for accurate determination of Echinococcus species such as the ones proposed in this work.
本研究旨在通过结合经典和替代分子工具，确定阿根廷和巴西流行地区中间宿主、终末宿主及人类分离株中的细粒棘球绦虫复合种及其基因型，包括那些尚无分子数据的地区。共从人类、天然中间宿主和终末宿主中分离出227个样本。对细胞色素c氧化酶亚基I基因片段进行扩增，并采用AluI酶切分析、高分辨率熔解分析（HRM）和DNA测序相结合的方法来确定棘球绦虫的种类/基因型。在绵羊（n = 35）、牛（n = 67）和狗（n = 5）中发现了狭义细粒棘球绦虫（G1）；在人类（n = 3）和牛（n = 108）中发现了奥氏棘球绦虫（G5）；在人类（n = 2）中发现了加拿大棘球绦虫（G6），在猪（n = 7）中发现了加拿大棘球绦虫（G7）。我们首次报道了在阿根廷圣胡安省和卡塔马卡省的人类中存在奥氏棘球绦虫（G5）和加拿大棘球绦虫（G6），以及在阿根廷科尔多瓦省的猪中存在加拿大棘球绦虫（G7）。在本研究中，我们通过分析来自终末宿主和中间宿主（包括流行地区信息匮乏或没有相关信息的人类）的多个分离株，拓宽了南美洲细粒棘球绦虫复合种的分子流行病学研究。同一地区和宿主物种中存在不同的种类/基因型，这凸显了需要快速且特异的技术来准确鉴定棘球绦虫种类，比如本研究中所提出的技术。
