Boufana Belgees, Lahmar Samia, Rebaï Waël, Ben Safta Zoubeir, Jebabli Leïla, Ammar Adel, Kachti Mahmoud, Aouadi Soufia, Craig Philip S
Ecosystems and Environment Research Centre, School of Environment and Life Sciences, University of Salford, Salford, M5 4WT, UK
Parasitology Laboratory, National School of Veterinary Medicine, Sidi Thabet, Tunisia.
Trans R Soc Trop Med Hyg. 2014 Nov;108(11):706-14. doi: 10.1093/trstmh/tru138. Epub 2014 Sep 11.
The species/genotypes of Echinococcus infecting a range of intermediate, canid and human hosts were examined as well as the intraspecific variation and population structure of Echinococcus granulosus sensu lato (s.l.) within these hosts.
A total of 174 Echinococcus isolates from humans and ungulate intermediate hosts and adult tapeworms from dogs and jackals were used. Genomic DNA was used to amplify a fragment within a mitochondrial gene and a nuclear gene, coding for cytochrome c oxidase subunit 1 (cox1; 828 bp) and elongation factor 1-alpha (ef1a; 656 bp), respectively.
E. granulosus sensu stricto was identified from all host species examined, E. canadensis (G6) in a camel and, for the first time, fertile cysts of E. granulosus (s.s.) and E. equinus in equids (donkeys) and E. granulosus (s.s.) from wild boars and goats. Considerable genetic variation was seen only for the cox1 sequences of E. granulosus (s.s.). The pairwise fixation index (Fst) for cox1 E. granulosus (s.s.) sequences from donkeys was high and was statistically significant compared with that of E. granulosus populations from other intermediate hosts. A single haplotype (EqTu01) was identified for the cox1 nucleotide sequences of E. equinus.
The role of donkeys in the epidemiology of echinococcosis in Tunisia requires further investigation.
对感染一系列中间宿主、犬科动物宿主和人类宿主的棘球绦虫的种类/基因型进行了研究，同时也研究了细粒棘球绦虫复合种（s.l.）在这些宿主体内的种内变异和种群结构。
共使用了174株来自人类和有蹄类中间宿主的棘球绦虫分离株以及来自狗和豺的成虫绦虫。基因组DNA用于扩增线粒体基因和核基因内的一个片段，分别编码细胞色素c氧化酶亚基1（cox1；828 bp）和延伸因子1-α（ef1a；656 bp）。
在所检查的所有宿主物种中均鉴定出狭义细粒棘球绦虫，在骆驼中鉴定出加拿大棘球绦虫（G6），首次在马属动物（驴）中发现了狭义细粒棘球绦虫和马棘球绦虫的可育囊肿，以及来自野猪和山羊的狭义细粒棘球绦虫。仅在狭义细粒棘球绦虫的cox1序列中观察到相当大的遗传变异。驴源狭义细粒棘球绦虫cox1序列的成对固定指数（Fst）较高，与来自其他中间宿主的细粒棘球绦虫种群相比具有统计学意义。马棘球绦虫的cox1核苷酸序列鉴定出一个单倍型（EqTu01）。
驴在突尼斯棘球蚴病流行病学中的作用需要进一步研究。
