Rajasekharan Satish Kumar, Ramesh Samiraj, Satish Ann Susan, Lee Jintae
School of Chemical Engineering, Yeungnam University, Gyeongsan, 38541, Republic of Korea.
Centre for Research and Development, PRIST University, Thanjavur 614-904, Tamil Nadu, India.
J Microbiol Biotechnol. 2017 Mar 28;27(3):542-551. doi: 10.4014/jmb.1609.09043.
Small phytochemicals have been successfully adopted as antibacterial chemotherapies and are being increasingly viewed as potential antibiofilm agents. Some of these molecules are known to repress biofilm and toxin production by certain bacterial and yeast pathogens, but information is lacking with regard to the genes allied with biofilm formation. The present study was performed to investigate the inhibitory effect of burdock root extract (BRE) and of chlorogenic acid (CGA; a component of BRE) on clinical isolates of . BRE and CGA exhibited significant antibiofilm activity against without inflicting any harm to its planktonic counterparts. In vitro assays supported the β-lactamase inhibitory effect of CGA and BRE while in silico docking showed that CGA bound strongly with the active sites of sulfhydryl-variable-1 β-lactamase. Furthermore, the mRNA transcript levels of two biofilm-associated genes (type 3 fimbriae and trehalose-6-phosphate hydrolase ) were significantly downregulated in CGA- and BRE-treated samples. In addition, CGA inhibited biofilm formation by and without affecting their planktonic cell growth. These findings show that BRE and its component CGA have potential use in antibiofilm strategies against persistent infections.
小型植物化学物质已成功用作抗菌化疗药物，并越来越被视为潜在的抗生物膜剂。已知其中一些分子可抑制某些细菌和酵母病原体的生物膜及毒素产生，但关于与生物膜形成相关的基因的信息却很缺乏。本研究旨在调查牛蒡根提取物（BRE）和绿原酸（CGA；BRE的一种成分）对……临床分离株的抑制作用。BRE和CGA对……表现出显著的抗生物膜活性，且对其浮游菌无任何损害。体外试验支持了CGA和BRE的β-内酰胺酶抑制作用，而计算机模拟对接显示CGA与巯基可变-1β-内酰胺酶的活性位点紧密结合。此外，在经CGA和BRE处理的样本中，两个生物膜相关基因（3型菌毛和海藻糖-6-磷酸水解酶）的mRNA转录水平显著下调。此外，CGA抑制了……和……的生物膜形成，而不影响它们的浮游细胞生长。这些发现表明，BRE及其成分CGA在针对持续性……感染的抗生物膜策略中具有潜在用途。
