基于微小RNA筛选与c-Myc的合成致死相互作用
MicroRNA-based screens for synthetic lethal interactions with c-Myc.
作者信息
Li Youjun, Zhu Yahui, Prochownik Edward V
机构信息
College of Life Sciences, Wuhan University, Wuhan 430072, China; Medical Research Institute, Wuhan University, Wuhan 430071, China.
College of Life Sciences, Wuhan University, Wuhan 430072, China.
出版信息
RNA Dis. 2016;3(3). doi: 10.14800/rd.1330. Epub 2016 May 30.
Abstract
microRNAs (miRs) are small, non-coding RNAs, which play crucial roles in the development and progression of human cancer. Given that miRs are stable, easy to synthetize and readily introduced into cells, they have been viewed as having potential therapeutic benefit in cancer. c-Myc (Myc) is one of the most commonly deregulated oncogenic transcription factors and has important roles in the pathogenesis of cancer, thus making it an important, albeit elusive therapeutic target. Here we review the miRs that have been identified as being both positive and negative targets for Myc and how these participate in the complex phenotypes that arise as a result of Myc-driven transformation. We also discussseveral recent reports of Myc-synthetic lethal interactions with miRs.These highlight the importance and complexity of miRs in Myc-mediated biological functions and the opportunities for Myc-driven human cancer therapies.
摘要
微小RNA（miRs）是一类小的非编码RNA，在人类癌症的发生和发展中起着关键作用。鉴于miRs稳定、易于合成且易于导入细胞，它们被认为在癌症治疗中具有潜在益处。c-Myc（Myc）是最常失调的致癌转录因子之一，在癌症发病机制中起重要作用，因此它是一个重要但难以捉摸的治疗靶点。在这里，我们综述了已被确定为Myc的正向和负向靶点的miRs，以及这些miRs如何参与由Myc驱动的转化所产生的复杂表型。我们还讨论了最近几篇关于Myc与miRs合成致死相互作用的报道。这些报道突出了miRs在Myc介导的生物学功能中的重要性和复杂性，以及Myc驱动的人类癌症治疗的机会。
相似文献
1
MicroRNA-based screens for synthetic lethal interactions with c-Myc.基于微小RNA筛选与c-Myc的合成致死相互作用
RNA Dis. 2016;3(3). doi: 10.14800/rd.1330. Epub 2016 May 30.
2
Splice-switch oligonucleotide-based combinatorial platform prioritizes synthetic lethal targets CHK1 and BRD4 against MYC-driven hepatocellular carcinoma.基于剪接转换寡核苷酸的组合平台确定了针对MYC驱动的肝细胞癌的合成致死靶点CHK1和BRD4。
Bioeng Transl Med. 2022 Sep 3;8(1):e10363. doi: 10.1002/btm2.10363. eCollection 2023 Jan.
3
MicroRNA functional network in pancreatic cancer: from biology to biomarkers of disease.胰腺癌中的 microRNA 功能网络：从生物学到疾病生物标志物。
J Biosci. 2011 Aug;36(3):481-91. doi: 10.1007/s12038-011-9083-4.
4
The Quest for Targets Executing MYC-Dependent Cell Transformation.对执行MYC依赖性细胞转化的靶点的探索。
Front Oncol. 2016 Jun 2;6:132. doi: 10.3389/fonc.2016.00132. eCollection 2016.
5
Capitalizing on Synthetic Lethality of MYC to Treat Cancer in the Digital Age.利用 MYC 的合成致死性在数字时代治疗癌症。
Trends Pharmacol Sci. 2021 Mar;42(3):166-182. doi: 10.1016/j.tips.2020.11.014. Epub 2021 Jan 6.
6
Synthetic lethal screens as a means to understand and treat MYC-driven cancers.合成致死筛选作为一种理解和治疗 MYC 驱动型癌症的方法。
Cold Spring Harb Perspect Med. 2014 Mar 1;4(3):a014209. doi: 10.1101/cshperspect.a014209.
7
Synthetic microRNA cassette dosing: pharmacokinetics, tissue distribution and bioactivity.合成 microRNA 盒给药：药代动力学、组织分布和生物活性。
Mol Pharm. 2012 Jun 4;9(6):1638-44. doi: 10.1021/mp2006483. Epub 2012 May 23.
8
MicroRNAs and oncogenic transcriptional regulatory networks controlling metabolic reprogramming in cancers.微小RNA与控制癌症代谢重编程的致癌转录调控网络
Comput Struct Biotechnol J. 2016 Jun 4;14:223-33. doi: 10.1016/j.csbj.2016.05.005. eCollection 2016.
9
Interactions between microRNAs and long non-coding RNAs in cardiac development and repair.miRNAs 与长非编码 RNA 在心脏发育和修复中的相互作用。
Pharmacol Res. 2018 Jan;127:58-66. doi: 10.1016/j.phrs.2017.05.029. Epub 2017 Jun 17.
10
Short GC-rich RNA similar to miR 1909 and 1915 folds in silico with the 5'-UTR and ORF of Notch and responders: potential for the elimination of cancer stem cells.短 GC 富集 RNA 类似于 miR-1909 和 1915，可在 Notch 的 5'-UTR 和 ORF 及其反应元件上发生折叠：消除癌症干细胞的潜力。
Oncol Rep. 2010 Dec;24(6):1443-53. doi: 10.3892/or_00001004.
引用本文的文献
1
hsa-let-7b-5p/TMPO-AS1-mediated ceRNA networks are linked to poor prognosis for lung cancer patients with FOXM1/MAD2L1 axis.hsa-let-7b-5p/TMPO-AS1介导的ceRNA网络与FOXM1/MAD2L1轴相关的肺癌患者预后不良有关。
Discov Oncol. 2025 May 30;16(1):953. doi: 10.1007/s12672-025-02631-y.
2
The Role of Non-Coding RNAs in MYC-Mediated Metabolic Regulation: Feedback Loops and Interactions.非编码RNA在MYC介导的代谢调控中的作用：反馈回路与相互作用
Noncoding RNA. 2025 Mar 18;11(2):27. doi: 10.3390/ncrna11020027.
3
Regulation of DNA Damage Response and Homologous Recombination Repair by microRNA in Human Cells Exposed to Ionizing Radiation.电离辐射暴露的人类细胞中 microRNA 对 DNA 损伤反应和同源重组修复的调控
Cancers (Basel). 2020 Jul 8;12(7):1838. doi: 10.3390/cancers12071838.
4
Interspecies analysis of MYC targets identifies tRNA synthetases as mediators of growth and survival in MYC-overexpressing cells.种间分析 MYC 靶标鉴定 tRNA 合成酶作为 MYC 过表达细胞中生长和存活的介质。
Proc Natl Acad Sci U S A. 2019 Jul 16;116(29):14614-14619. doi: 10.1073/pnas.1821863116. Epub 2019 Jul 1.
5
Different EPHX1 methylation levels in promoter area between carbamazepine-resistant epilepsy group and carbamazepine-sensitive epilepsy group in Chinese population.中国人群中卡马西平耐药癫痫组和卡马西平敏感癫痫组启动子区域EPHX1甲基化水平不同。
BMC Neurol. 2019 Jun 4;19(1):114. doi: 10.1186/s12883-019-1308-4.
6
Complex role of miR-130a-3p and miR-148a-3p balance on drug resistance and tumor biology in esophageal squamous cell carcinoma.miR-130a-3p 和 miR-148a-3p 平衡在食管鳞癌耐药和肿瘤生物学中的复杂作用。
Sci Rep. 2018 Dec 3;8(1):17553. doi: 10.1038/s41598-018-35799-1.
7
Identification of microRNAs associated with glioma diagnosis and prognosis.与神经胶质瘤诊断和预后相关的微小RNA的鉴定。
Oncotarget. 2017 Apr 18;8(16):26394-26403. doi: 10.18632/oncotarget.14445.
8
New differentially expressed genes and differential DNA methylation underlying refractory epilepsy.难治性癫痫潜在的新差异表达基因和DNA甲基化差异
Oncotarget. 2016 Dec 27;7(52):87402-87416. doi: 10.18632/oncotarget.13642.
本文引用的文献
1
Role of Signal Regulatory Protein α in Arsenic Trioxide-induced Promyelocytic Leukemia Cell Apoptosis.信号调节蛋白α在三氧化二砷诱导早幼粒细胞白血病细胞凋亡中的作用
Sci Rep. 2016 Mar 24;6:23710. doi: 10.1038/srep23710.
2
microRNA-129-5p, a c-Myc negative target, affects hepatocellular carcinoma progression by blocking the Warburg effect.微小RNA-129-5p是一种c-Myc负向靶标，通过阻断瓦伯格效应影响肝细胞癌进展。
J Mol Cell Biol. 2016 Oct 1;8(5):400-410. doi: 10.1093/jmcb/mjw010.
3
miR-221 attenuates the osteogenic differentiation of human annulus fibrosus cells.微小RNA-221减弱人纤维环细胞的成骨分化。
Spine J. 2016 Jul;16(7):896-904. doi: 10.1016/j.spinee.2016.03.026. Epub 2016 Mar 17.
4
CDK4 and miR-15a comprise an abnormal automodulatory feedback loop stimulating the pathogenesis and inducing chemotherapy resistance in nasopharyngeal carcinoma.细胞周期蛋白依赖性激酶4（CDK4）和微小RNA-15a（miR-15a）构成一个异常的自调节反馈环，刺激鼻咽癌的发病机制并诱导化疗耐药性。
BMC Cancer. 2016 Mar 18;16:238. doi: 10.1186/s12885-016-2277-2.
5
miR-203 and miR-221 regulate SOCS1 and SOCS3 in essential thrombocythemia.微小RNA-203和微小RNA-221在原发性血小板增多症中调节细胞因子信号传导抑制因子1和细胞因子信号传导抑制因子3。
Blood Cancer J. 2016 Mar 18;6(3):e406. doi: 10.1038/bcj.2016.10.
6
E2F7 regulates transcription and maturation of multiple microRNAs to restrain cell proliferation.E2F7通过调控多种微小RNA的转录和成熟来抑制细胞增殖。
Nucleic Acids Res. 2016 Jul 8;44(12):5557-5570. doi: 10.1093/nar/gkw146. Epub 2016 Mar 9.
7
microRNA-206 impairs c-Myc-driven cancer in a synthetic lethal manner by directly inhibiting MAP3K13.微小RNA-206通过直接抑制丝裂原活化蛋白激酶激酶激酶13，以合成致死的方式损害c-Myc驱动的癌症。
Oncotarget. 2016 Mar 29;7(13):16409-19. doi: 10.18632/oncotarget.7653.
8
MiR-494 Inhibits Epithelial Ovarian Cancer Growth by Targeting c-Myc.微小RNA-494通过靶向c-Myc抑制上皮性卵巢癌生长。
Med Sci Monit. 2016 Feb 24;22:617-24. doi: 10.12659/msm.897288.
9
MiR-449a Affects Epithelial Proliferation during the Pseudoglandular and Canalicular Phases of Avian and Mammal Lung Development.微小RNA-449a在鸟类和哺乳动物肺发育的假腺期和小管期影响上皮细胞增殖。
PLoS One. 2016 Feb 18;11(2):e0149425. doi: 10.1371/journal.pone.0149425. eCollection 2016.
10
Identification of MLL-fusion/MYC⊣miR-26⊣TET1 signaling circuit in MLL-rearranged leukemia.MLL重排白血病中MLL融合/MYC⊣miR-26⊣TET1信号通路的鉴定
Cancer Lett. 2016 Mar 28;372(2):157-65. doi: 10.1016/j.canlet.2015.12.032. Epub 2016 Jan 11.
Zotero 插件