Lvov D K, Leonova G N, Gromashevsky V L, Skvortsova T M, Shestakov V I, Belikova N P, Berezina L K, Gofman Y P, Klimenko S M, Safonov A V, Sazonov A A, Zakaryan V A
Acta Virol. 1978 May;22(3):249-52.
Strain LEIV-776P, possessing no haemagglutinating activity was isolated in 1971 in the Primorie region (U.S.S.R.) from Haemaphysalis longicornis Neumann 1091 tick. Complement fixation (CF) tests revealed no antigenic relationship with 24 antigenic groups of arboviruses or 21 ungrouped viruses isolated from ticks. The virus isolate contains RNA and is relatively sensitive to ether and sodium deoxycholate; it is pathogenic for suckling mice and two-week-old white mice by the intracerebral route, and replicates in primary cultures of chick and duck fibroblasts and green monkey kidneys and in a continuous line of pig embryo kidney cells without any cytopathic effect. According to electron microscopy, its size is 90-110 nm. Its morphological properties permit to classify it as a member of the family Bunyaviridae.
毒株LEIV - 776P于1971年在前苏联滨海边疆区从长角血蜱（Haemaphysalis longicornis Neumann 1091）中分离得到，它不具备血凝活性。补体结合（CF）试验表明，该毒株与从蜱中分离出的24个虫媒病毒抗原组或21种未分类病毒均无抗原关系。该病毒分离株含有RNA，对乙醚和脱氧胆酸钠相对敏感；通过脑内途径对乳鼠和两周龄小白鼠具有致病性，并能在鸡和鸭成纤维细胞、绿猴肾原代培养物以及猪胚胎肾细胞连续系中复制，且无任何细胞病变效应。根据电子显微镜观察，其大小为90 - 110纳米。其形态学特征使其可被归类为布尼亚病毒科的成员。
