太平洋鳕鱼（大头鳕）骨钙结合肽对大鼠钙生物利用度的影响。
Effect of calcium-binding peptide from Pacific cod (Gadus macrocephalus) bone on calcium bioavailability in rats.
机构信息
College of Food Science and Engineering, Ocean University of China, No. 5, Yu Shan Road, Qingdao, Shandong Province 266003, PR China.
College of Food Science and Engineering, Ocean University of China, No. 5, Yu Shan Road, Qingdao, Shandong Province 266003, PR China.
出版信息
Food Chem. 2017 Apr 15;221:373-378. doi: 10.1016/j.foodchem.2016.10.078. Epub 2016 Oct 20.
Bone collagen peptide with high affinity to Ca was extracted from Pacific cod (Gadus macrocephalus) bone. FTIR spectra of calcium-binding bone collagen peptide showed that band at 3381cm shifted to 3361cm, 1455cm moved to 1411cm, and amide II became deeper valley, compared with that of bone collagen peptide. This peptide was sequenced by Q-TOF-MS and sequences of Gly-Pro-Glu-Gly, Gly-Glu-Lys, Gly-Pro-Leu-Gly and Gly-Leu-Pro-Gly appeared repeatedly in some peptides. From SEM, after chelated with calcium, the loose and porous structure turned into granular structure. From the animal experiment, Ca apparent absorption rate, Ca retention rate and femur Ca content of calcium-binding bone collagen peptide group were significantly higher than those of model and CaCO groups (P<0.05), while serum ALP was significantly lower than model group (P<0.05) and similar to control group. The results suggested that calcium-binding bone collagen peptide could improve bioavailability of Ca and thus prevented Ca deficiency.
从太平洋鳕鱼（大头鳕）骨中提取了对钙具有高亲和力的骨胶原蛋白肽。钙结合骨胶原蛋白肽的傅里叶变换红外光谱（FTIR）显示，与骨胶原蛋白肽相比，3381cm 处的带移至 3361cm，1455cm 移至 1411cm，酰胺 II 变得更深。通过 Q-TOF-MS 对该肽进行测序，在一些肽中反复出现 Gly-Pro-Glu-Gly、Gly-Glu-Lys、Gly-Pro-Leu-Gly 和 Gly-Leu-Pro-Gly 序列。从 SEM 来看，螯合钙后，疏松多孔的结构变成了颗粒状结构。从动物实验来看，钙结合骨胶原蛋白肽组的钙表观吸收率、钙保留率和股骨钙含量均明显高于模型组和碳酸钙组（P<0.05），而血清碱性磷酸酶（ALP）明显低于模型组（P<0.05），与对照组相似。结果表明，钙结合骨胶原蛋白肽可以提高钙的生物利用度，从而预防钙缺乏症。
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