Dipartimento di Chimica, Sapienza University of Rome, 00185 Rome, Italy.
Department of Pharmacy, "G. D'Annunzio" University of Chieti-Pescara, 66100 Chieti, Italy.
Food Chem. 2017 Apr 15;221:855-863. doi: 10.1016/j.foodchem.2016.11.086. Epub 2016 Nov 19.
A microwave-assisted extraction method was optimised for the recovery of bioactive compounds from Crocus sativus L. stigmas with the use of water/ethanol mixture. HPLC-DAD was employed to evaluate the extraction parameters, in particular, solvent type and volume, and the duration of the procedure. Microwave-assisted extraction enhanced the recovery of the active principles, limiting extraction time and solvent waste. Moreover, NIR experiments were performed in order to compare spectra in pseudo-absorbance of Saffron samples with different geographical origins through the application of the chemometric techniques. Moreover, the biological evaluation of crocin 1, safranal and its semisynthetic derivatives as selective inhibitors of five isoforms of human carbonic anhydrase was also explored.
采用微波辅助提取法，利用水/乙醇混合物从藏红花柱头中提取生物活性化合物。采用 HPLC-DAD 评估提取参数，特别是溶剂类型和体积以及提取程序的持续时间。微波辅助提取可提高活性成分的回收率，同时限制提取时间和溶剂浪费。此外，还进行了 NIR 实验，通过应用化学计量技术，比较了来自不同地理来源的藏红花样品的伪吸收光谱。此外，还研究了西红花苷 1、西红花醛及其半合成衍生物作为五种人碳酸酐酶同工酶选择性抑制剂的生物活性。
