亚临界水萃取蛹虫草多糖的结构解析及免疫活性研究。
Structural elucidation and immunostimulatory activity of polysaccharide isolated by subcritical water extraction from Cordyceps militaris.
机构信息
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
出版信息
Carbohydr Polym. 2017 Feb 10;157:794-802. doi: 10.1016/j.carbpol.2016.10.066. Epub 2016 Oct 23.
Water-soluble polysaccharides were obtained from Cordyceps militaris (C. militaris) (CMP) by subcritical water extraction (SWE). Two polysaccharides fractions, CMP-W1 and CMP-S1, were isolated from CMP using DEAE-52 cellulose and Sephadex G-150 column chromatography. The structural characteristics of CMP-W1 and CMP-S1 were investigated. The results showed that the molecular weight of CMP-W1 and CMP-S1 are 3.66×105Da and 4.60×105Da, respectively, and both of them were heteropolysaccharides composed of d-mannose, d-glucose, d-galactose with the molar ratios of 2.84:1:1.29 and 2.05:1:1.09, respectively. FT-IR spectra analysis suggested that CMP-W1 and CMP-S1 belonged to pyranose form sugar and protein free. For immunostimulatory activity assay in vitro, CMP-W1 and CMP-S1 significantly promoted lymphatic spleen cell proliferation of mice. Therefore, the polysaccharides obtained from C. militaris by SWE can be used as potential natural immunostimulant in functional foods or medicine.
采用亚临界水萃取法（SWE）从蛹虫草（Cordyceps militaris）（CMP）中提取水溶性多糖。使用 DEAE-52 纤维素和 Sephadex G-150 柱色谱法从 CMP 中分离出两种多糖级分，CMP-W1 和 CMP-S1。研究了 CMP-W1 和 CMP-S1 的结构特征。结果表明，CMP-W1 和 CMP-S1 的分子量分别为 3.66×105Da 和 4.60×105Da，均为杂多糖，由 d-甘露糖、d-葡萄糖、d-半乳糖组成，摩尔比分别为 2.84:1:1.29 和 2.05:1:1.09。FT-IR 光谱分析表明，CMP-W1 和 CMP-S1 属于吡喃糖形式的糖，不含蛋白质。体外免疫刺激活性试验表明，CMP-W1 和 CMP-S1 能显著促进小鼠脾淋巴细胞增殖。因此，SWE 从蛹虫草中得到的多糖可用作功能性食品或药物中潜在的天然免疫刺激物。
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