Department of Biological Sciences, Faculty of Science, University of Calgary, Calgary, Alberta, Canada.
Department of Biological Sciences, Faculty of Science, University of Calgary, Calgary, Alberta, Canada.
Trends Parasitol. 2017 Feb;33(2):113-127. doi: 10.1016/j.pt.2016.11.007. Epub 2016 Dec 14.
Toxoplasma gondii is an intensely studied protozoan parasite. It is also used as a model organism to research additional clinically relevant human and veterinary parasites due to ease of in vitro culture and genetic manipulation. Recently, it has been developed as a model of inflammatory bowel disease, due to their similar pathologies. However, researchers vary widely in how they use T. gondii, which makes study comparisons and interpretation difficult. The aim of this review is to provide researchers with a tool to: (i) determine the appropriateness of the different T. gondii models to their research, (ii) interpret results from the wide range of study conditions, and (iii) consider new advances in technology which could improve or refine their experimental setup.
刚地弓形虫是一种研究得非常深入的原生动物寄生虫。由于其易于体外培养和遗传操作，它也被用作研究其他与临床相关的人类和兽医寄生虫的模式生物。最近，由于其相似的病理学，它已被开发为炎症性肠病的模型。然而，研究人员在使用刚地弓形虫方面差异很大，这使得研究比较和解释变得困难。本综述的目的是为研究人员提供一个工具：（i）确定不同的刚地弓形虫模型是否适合他们的研究，（ii）解释来自广泛研究条件的结果，以及（iii）考虑新技术的新进展，这些进展可以改进或完善他们的实验设置。
