Ross D W
Nouv Rev Fr Hematol (1978). 1978 Jun 10;20(2):297-302.
The use of electronic pulse spectroscopy to determine the volume distribution of a white blood cell fraction isolated by sedimentation from anticoagulated whole blood permits a rapid quantitative measure of size and dispersion of white blood cells in suspension. In patients without apparent hematologic diseases the white cell volume distribution presents as a broad peak. In patients with a large percentage of circulatory malignant cells, a single narrow volume peak is observed in acute leukemias and a broad and sometimes bimodal curve is observed in chronic leukemias. The volume of the leukemic cells is observed to shift in vivo after the start of effective chemotherapy as was predicted on the basis of a tissue culture model. The prognostic significance of the white cell volume distribution in leukemia and the use of a shift in size of leukemic cells after the start of therapy as a means of monitoring therapy are discussed.
利用电子脉冲光谱法来确定通过抗凝全血沉降分离出的白细胞组分的体积分布，能够快速定量测量悬浮液中白细胞的大小和分散度。在无明显血液学疾病的患者中，白细胞体积分布呈现为一个宽峰。在循环恶性细胞比例较高的患者中，急性白血病观察到单个窄体积峰，慢性白血病则观察到宽且有时为双峰的曲线。正如基于组织培养模型所预测的那样，有效化疗开始后，白血病细胞的体积在体内会发生变化。讨论了白血病中白细胞体积分布的预后意义以及将治疗开始后白血病细胞大小的变化作为监测治疗手段的应用。
