利用淀粉酶可控合成生物相容性银纳米颗粒的环保方法。
Environment friendly approach for size controllable synthesis of biocompatible Silver nanoparticles using diastase.
作者信息
Maddinedi Sireesh Babu, Mandal Badal Kumar, Anna Kiran Kumar
机构信息
The Key laboratory of Advanced Textile Materials and Manufacturing Technology of Ministry of Education, National Engineering Lab for Textile Fiber Materials and Processing Technology (Zhejiang), College of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou 310018, China; Trace Elements Speciation Research Laboratory, Department of Chemistry, School of Advanced Sciences, VIT University, Vellore 632014, India.
Trace Elements Speciation Research Laboratory, Department of Chemistry, School of Advanced Sciences, VIT University, Vellore 632014, India.
出版信息
Environ Toxicol Pharmacol. 2017 Jan;49:131-136. doi: 10.1016/j.etap.2016.11.019. Epub 2016 Nov 30.
A green, facile method for the size selective synthesis of silver nanoparticles (AgNPs) using diastase as green reducing and stabilizing agent is reported. The thiol groups present in the diastase are mainly responsible for the rapid reaction rate of silver nanoparticles synthesis. The variation in the size and morphology of AgNPs were studied by changing the pH of diastase. The prepared silver nanoparticles were characterized by using UV-vis, XRD, FTIR, TEM and SAED. The FTIR analysis revealed the stabilization of diastase molecules on the surface of AgNPs. Additionally, in-vitro cytotoxicity experiments concluded that the cytotoxicity of the as-synthesized AgNPs towards mouse fibroblast (3T3) cell lines is dose and size dependent. Furthermore, the present method is an alternative to the traditional chemical methods of size controlled AgNPs synthesis.
报道了一种绿色、简便的方法，该方法使用淀粉酶作为绿色还原剂和稳定剂，用于尺寸选择性合成银纳米颗粒（AgNPs）。淀粉酶中存在的巯基是银纳米颗粒合成反应速率快的主要原因。通过改变淀粉酶的pH值，研究了AgNPs尺寸和形态的变化。使用紫外可见光谱、X射线衍射、傅里叶变换红外光谱、透射电子显微镜和选区电子衍射对制备的银纳米颗粒进行了表征。傅里叶变换红外光谱分析表明淀粉酶分子在AgNPs表面得到了稳定。此外，体外细胞毒性实验得出结论，合成的AgNPs对小鼠成纤维细胞（3T3）细胞系的细胞毒性具有剂量和尺寸依赖性。此外，本方法是尺寸可控的AgNPs合成传统化学方法的一种替代方法。
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