分离的微血管：体外血脑屏障
Isolated microvessels: the blood-brain barrier in vitro.
作者信息
Hjelle J T, Baird-Lambert J, Cardinale G, Specor S, Udenfriend S
出版信息
Proc Natl Acad Sci U S A. 1978 Sep;75(9):4544-8. doi: 10.1073/pnas.75.9.4544.
Abstract
Isolated bovine retinal and brain microvessels, exhibiting a patent lumen, were used to study the contribution of the microvasculature to the blood-brain and blood-retina barriers. The diffusion marker, sucrose, was taken up slowly by the isolated microvessels in contrast to leucine, tyrosine, and valine which were taken up at a considerably faster rate. Uptake of leucine was temperature dependent but resistant to inhibition by ouabain and sodium azide. The large neutral amino acids exhibited stereospecificity and cross-competition for uptake by the isolated microvessels. The apparent Kms for uptake for tyrosine, leucine, and valine were III micron,133 micron, and 500 micron, respectively.
摘要
分离出的具有开放管腔的牛视网膜和脑微血管被用于研究微血管对血脑屏障和血视网膜屏障的作用。与亮氨酸、酪氨酸和缬氨酸相比，扩散标记物蔗糖被分离出的微血管缓慢摄取，亮氨酸、酪氨酸和缬氨酸的摄取速度要快得多。亮氨酸的摄取依赖于温度，但不受哇巴因和叠氮化钠的抑制。大中性氨基酸对分离出的微血管摄取表现出立体特异性和交叉竞争。酪氨酸、亮氨酸和缬氨酸摄取的表观米氏常数分别为111微摩尔、133微摩尔和500微摩尔。
相似文献
1
Isolated microvessels: the blood-brain barrier in vitro.分离的微血管：体外血脑屏障
Proc Natl Acad Sci U S A. 1978 Sep;75(9):4544-8. doi: 10.1073/pnas.75.9.4544.
2
Characteristics of the large neutral amino acid transport system of bovine brain microvessel endothelial cell monolayers.牛脑微血管内皮细胞单层的大中性氨基酸转运系统的特征
J Neurochem. 1986 Aug;47(2):484-8. doi: 10.1111/j.1471-4159.1986.tb04527.x.
3
Polarity of the blood-brain barrier: neutral amino acid transport into isolated brain capillaries.血脑屏障的极性：中性氨基酸转运至分离的脑毛细血管中。
Science. 1978 Oct 13;202(4364):225-7. doi: 10.1126/science.211586.
4
5
Identification of the insulin receptor of cerebral microvessels.
Am J Physiol. 1985 Jan;248(1 Pt 1):E115-25. doi: 10.1152/ajpendo.1985.248.1.E115.
6
Uptake of adenosine by isolated bovine cortex microvessels.腺苷在分离的牛脑皮质微血管中的摄取。
Neurochem Res. 1983 Nov;8(11):1459-69. doi: 10.1007/BF00965001.
7
Pericyte abundance affects sucrose permeability in cultures of rat brain microvascular endothelial cells.周细胞丰度影响大鼠脑微血管内皮细胞培养物中的蔗糖通透性。
Brain Res. 2005 Jul 5;1049(1):8-14. doi: 10.1016/j.brainres.2005.04.054.
8
Na+-dependent transport of large neutral amino acids occurs at the abluminal membrane of the blood-brain barrier.大中性氨基酸的钠依赖性转运发生在血脑屏障的无腔膜处。
Am J Physiol Endocrinol Metab. 2003 Dec;285(6):E1167-73. doi: 10.1152/ajpendo.00193.2003. Epub 2003 Aug 21.
9
Blood-brain barrier permeability to excitatory amino acids.血脑屏障对兴奋性氨基酸的通透性。
Adv Exp Med Biol. 1986;203:191-8. doi: 10.1007/978-1-4684-7971-3_14.
10
Combination of hypoxia/aglycemia compromises in vitro blood-brain barrier integrity.缺氧/无糖血症的联合作用会损害体外血脑屏障的完整性。
J Pharmacol Exp Ther. 1999 May;289(2):668-75.
引用本文的文献
1
Dietary supplementation of valine, isoleucine, and tryptophan may overcome the negative effects of excess leucine in diets for weanling pigs containing corn fermented protein.在含玉米发酵蛋白的断奶仔猪日粮中，补充缬氨酸、异亮氨酸和色氨酸可能会克服过量亮氨酸带来的负面影响。
J Anim Sci Biotechnol. 2024 Sep 10;15(1):125. doi: 10.1186/s40104-024-01082-9.
2
The biological functions and metabolic pathways of valine in swine.缬氨酸在猪体内的生物学功能及代谢途径
J Anim Sci Biotechnol. 2023 Oct 7;14(1):135. doi: 10.1186/s40104-023-00927-z.
3
Improving performance of finishing pigs with added valine, isoleucine, and tryptophan: validating a meta-analysis model.添加缬氨酸、异亮氨酸和色氨酸可提高育肥猪的性能：验证荟萃分析模型。
J Anim Sci. 2021 Jan 1;99(1). doi: 10.1093/jas/skab006.
4
5
The Isolated Brain Microvessel: A Versatile Experimental Model of the Blood-Brain Barrier.孤立脑微血管：血脑屏障的通用实验模型。
Front Physiol. 2020 May 7;11:398. doi: 10.3389/fphys.2020.00398. eCollection 2020.
6
Meta-regression analysis to predict the influence of branched-chain and large neutral amino acids on growth performance of pigs1.元回归分析预测支链和大中性氨基酸对猪生长性能的影响 1 。
J Anim Sci. 2019 May 30;97(6):2505-2514. doi: 10.1093/jas/skz118.
7
Purification of Mouse Brain Vessels.小鼠脑血管的纯化
J Vis Exp. 2015 Nov 10(105):e53208. doi: 10.3791/53208.
8
Synergism between different transport systems stimulates the uptake of neutral amino acids by isolated brain microvessels.不同转运系统间的协同作用可刺激分离的脑微血管摄取中性氨基酸。
Amino Acids. 1992 Feb;2(1-2):13-23. doi: 10.1007/BF00806074.
9
Transport across the primate blood-brain barrier of a genetically engineered chimeric monoclonal antibody to the human insulin receptor.一种针对人胰岛素受体的基因工程嵌合单克隆抗体在灵长类动物血脑屏障中的转运。
Pharm Res. 2000 Mar;17(3):266-74. doi: 10.1023/a:1007592720793.
10
Blood-brain barrier transport of reduced folic acid.还原型叶酸的血脑屏障转运
Pharm Res. 1999 Mar;16(3):415-9. doi: 10.1023/a:1018829920158.
本文引用的文献
1
The brain barrier system--II. Uptake and transport of amino acids by the brain.脑屏障系统——II. 大脑对氨基酸的摄取与转运
J Neurochem. 1961 Dec;8:216-25. doi: 10.1111/j.1471-4159.1961.tb13545.x.
2
ISOLATION OF GAMMA-GLUTAMYL TRANSPEPTIDASE FROM HOG KIDNEY.从猪肾中分离γ-谷氨酰转肽酶
J Biol Chem. 1965 Jan;240:338-47.
3
4
Uptake of tyrosine by rat brain in vivo.大鼠脑在体内对酪氨酸的摄取。
J Biol Chem. 1960 Jul;235:2075-9.
5
Dopamine formation in brain capillaries--an enzymic blood-brain barrier mechanism.脑毛细血管中的多巴胺生成——一种酶促血脑屏障机制。
J Neurochem. 1967 May;14(5):547-50. doi: 10.1111/j.1471-4159.1967.tb09553.x.
6
The localization of monoaminergic blood-brain barrier mechanisms.单胺能血脑屏障机制的定位
Pharmacol Rev. 1966 Mar;18(1):369-85.
7
The use of N-methylation to direct route of mediated transport of amino acids.利用N-甲基化来指导氨基酸介导转运的途径。
J Biol Chem. 1965 Sep;240(9):3609-16.
8
Junctions between intimately apposed cell membranes in the vertebrate brain.脊椎动物大脑中紧密相邻细胞膜之间的连接。
J Cell Biol. 1969 Mar;40(3):648-77. doi: 10.1083/jcb.40.3.648.
9
The uptake of various amino acids by the mouse brain in vivo.小鼠大脑在体内对各种氨基酸的摄取。
Brain Res. 1971 Jun 4;29(1):85-99. doi: 10.1016/0006-8993(71)90419-7.
10
Passage into the rat brain of dopa and dopamine injected into the lateral ventricle.注入侧脑室的多巴和多巴胺进入大鼠脑内。
Br J Pharmacol. 1971 Sep;43(1):32-8. doi: 10.1111/j.1476-5381.1971.tb07154.x.
Zotero 插件