Haeggblom Linnea, Franzén Joar, Näsman Anders
Department of oncology and pathology, Karolinska Institutet, Stockholm, Sweden.
J Clin Pathol. 2017 May;70(5):451-453. doi: 10.1136/jclinpath-2016-204197. Epub 2016 Dec 19.
Keratoacanthomas (KA) and Spitz naevus (SN) are both lesions with unknown aetiology; therefore, the possibility of a viral involvement, more specifically the involvement of human polyomaviruses (HPyV), was investigated. In total, 22 cases of KA and 25 cases of SN were tested for the presence of HPyVs. DNA was extracted and amplified by multiplex PCR and thereafter tested with a multiplex bead-based assay for HPyVs (BKPyV, JCPyV, KIPyV, WUPyV, MCPyV, TSPyV, HPyV6, 7 and 9) and two primate viruses (SV40 and LPyV). HPyV DNA was found in 20 of the 47 lesions. There was no significant difference in HPyV DNA detection frequency between patients diagnosed with KA and patients diagnosed with SN, nor any over-representation of a specific HPyV type in any of the two patient categories. In conclusion, evidence for a specific aetiological role of any of the above tested HPyVs in either KA or SN was not disclosed.
角化棘皮瘤（KA）和斯皮茨痣（SN）均为病因不明的病变；因此，研究了病毒感染的可能性，更具体地说是人多瘤病毒（HPyV）感染的可能性。总共对22例KA和25例SN进行了HPyV检测。提取DNA并通过多重PCR进行扩增，然后用基于多重微珠的检测方法检测HPyV（BKPyV、JCPyV、KIPyV、WUPyV、MCPyV、TSPyV、HPyV6、7和9）以及两种灵长类病毒（SV40和LPyV）。在47个病变中的20个中发现了HPyV DNA。诊断为KA的患者和诊断为SN的患者在HPyV DNA检测频率上没有显著差异，且在任何一类患者中都没有特定HPyV类型的过度表现。总之，未发现上述检测的任何HPyV在KA或SN中具有特定病因作用的证据。
