Traversetti Lorenzo, Del Grosso Floriano, Malafoglia Valentina, Colasanti Marco, Ceschin Simona, Larsen Stefano, Scalici Massimiliano
Department of Sciences, University Rome Tre, V.le G. Marconi 446, Rome, 00146, Italy.
Institute for Research on Pain, ISAL-Foundation, Via San Salvador 204, Rimini, 47922, TP, Italy.
Ecotoxicology. 2017 Mar;26(2):184-195. doi: 10.1007/s10646-016-1753-4. Epub 2016 Dec 19.
The regenerative ability of Hydra vulgaris was tested as potential biomarker for the development of a new eco-toxicological index. The test is based on the regeneration rate and the aberration frequency of the columna (body and adhesive foot) after separation from head and tentacles by a bistoury. Particularly, 45 columnae were submerged in the rearing solution (that is Hydra medium) to have control, and 285 in potential contaminated waters to have treatments, collected from 19 sites along 10 rivers in central Italy. ANCOVA and chi-square tests were used to compare values from each site to a laboratory control. Subsequently the values on regeneration rate and aberration frequency were inserted in a double entry matrix, where the match of the two entries in the matrix provides the score of the proposed Teratogenic Risk Index (TRI). Each score corresponded to one of the 5 teratogenic risk classes, to which a risk level was associated: from 1 (no risk) to 5 (very high risk). On the whole, 32% of the studied sites were classified as no teratogenic risk while the remaining showed a variable risk level from low to very high. This study proposed for the first time an early warning system to detect the presence of teratogens in running waters, providing a rapid and cost-effective evaluation method. Therefore, TRI may contribute to initiate adequate measures to manage riverine habitats, and to monitor the running water teratogenic status. Specifically, this index may provide the opportunity to identify the disturbance sources and then to drive the decisions, together with competent authorities, on the catchment and landscape management and on the possible use of waters for urban, agricultural, and industrial activities, since they may show significant effects on the human health.
普通水螅的再生能力被作为一种潜在生物标志物进行测试，以开发一种新的生态毒理学指标。该测试基于用手术刀将柱体（身体和附着足）与头部和触手分离后的再生率和畸变频率。具体而言，45个柱体被浸没在饲养溶液（即水螅培养基）中作为对照，285个柱体被置于潜在受污染的水体中作为处理组，这些水体取自意大利中部10条河流沿线的19个地点。采用协方差分析和卡方检验将每个地点的值与实验室对照进行比较。随后，将再生率和畸变频率的值输入到一个双项矩阵中，矩阵中两个条目的匹配给出了所提议的致畸风险指数（TRI）的得分。每个得分对应5个致畸风险类别之一，每个类别都关联一个风险水平：从1（无风险）到5（非常高风险）。总体而言，32%的研究地点被归类为无致畸风险，而其余地点显示出从低到非常高的不同风险水平。这项研究首次提出了一种早期预警系统，用于检测流动水体中致畸剂的存在，提供了一种快速且经济高效的评估方法。因此，TRI可能有助于启动适当措施来管理河流栖息地，并监测流动水体的致畸状态。具体而言，该指数可能提供机会识别干扰源，然后与主管当局一起就集水区和景观管理以及水在城市、农业和工业活动中的可能用途做出决策，因为这些活动可能对人类健康产生重大影响。
