Andersen L, Attström R, Fejerskov O
Scand J Dent Res. 1978 Jul;86(4):237-47. doi: 10.1111/j.1600-0722.1978.tb00624.x.
The influence of experimental neutropenia on the wound healing process in standardized excisional palatal wounds was studied in 16 guinea pigs. Corresponding wounds in 15 normal animals were used as controls. Neutropenia was induced and maintained by daily injections of heterologous anti-neutrophil serum. One-micron sections from biopsies of palatal mucosa sampled 6, 24, 48 and 120 h after wounding were evaluated with special reference to the progress of epithelial wound repair and to the occurrence and location of inflammatory cells and bacteria. In animals treated with anti-serum, neutrophilic leukocytes disappeared from the wound cavity, whereas other inflammatory cells remained unchanged. Further bacterial invasion deep into the cavity was observed. The epithelial repair took place at a deeper level in the wounds of neutropenic animals compared with those of normal animals. The rate of re-epithelialization was the same in neutropenic and control wounds. The experiment indicates that the neutrophils in oral wounds provide a principal agency for host defense against microorganisms and probably thereby indirectly determine the direction and level of epithelial cell migration.
在16只豚鼠身上研究了实验性中性粒细胞减少对标准化腭部切除伤口愈合过程的影响。将15只正常动物的相应伤口作为对照。通过每日注射异源抗中性粒细胞血清诱导并维持中性粒细胞减少。对受伤后6、24、48和120小时采集的腭部黏膜活检组织制作的1微米切片进行评估，特别关注上皮伤口修复的进程以及炎症细胞和细菌的出现与位置。在用抗血清治疗的动物中，中性粒细胞从伤口腔中消失，而其他炎症细胞保持不变。观察到细菌进一步深入侵入伤口腔。与正常动物的伤口相比，中性粒细胞减少动物伤口的上皮修复发生在更深的层面。中性粒细胞减少伤口和对照伤口的上皮再形成速率相同。该实验表明，口腔伤口中的中性粒细胞是宿主抵御微生物的主要媒介，可能由此间接决定上皮细胞迁移的方向和水平。
