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用于快速体积成像的双光子激光扫描体视显微镜

Two-Photon Laser Scanning Stereomicroscopy for Fast Volumetric Imaging.

作者信息

Yang Yanlong, Yao Baoli, Lei Ming, Dan Dan, Li Runze, Horn Mark Van, Chen Xun, Li Yang, Ye Tong

机构信息

State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an, China.

Department of Radiology, Medical University of South Carolina, Charleston, South Carolina, United States of America.

出版信息

PLoS One. 2016 Dec 20;11(12):e0168885. doi: 10.1371/journal.pone.0168885. eCollection 2016.

DOI:10.1371/journal.pone.0168885
PMID:27997624
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5173245/
Abstract

Bessel beams have been successfully used in two-photon laser scanning fluorescence microscopy to extend the depth of field (EDF), which makes it possible to observe fast events volumetrically. However, the depth information is lost due to integration of fluorescence signals along the propagation direction. We describe the design and implementation of two-photon lasers scanning stereomicroscopy, which allows viewing dynamic processes in three-dimensional (3D) space stereoscopically in real-time with shutter glasses at the speed of 1.4 volumes per second. The depth information can be appreciated by human visual system or be recovered with correspondence algorithms for some cases.

摘要

贝塞尔光束已成功应用于双光子激光扫描荧光显微镜中以扩展景深（EDF），这使得对快速事件进行体视观察成为可能。然而，由于荧光信号沿传播方向的积分，深度信息会丢失。我们描述了双光子激光扫描体视显微镜的设计与实现，它允许使用快门眼镜以每秒1.4个容积的速度实时立体地观察三维（3D）空间中的动态过程。深度信息可以被人类视觉系统感知，或者在某些情况下通过对应算法恢复。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8ce/5173245/348c3bd8f23a/pone.0168885.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8ce/5173245/7a0bf25a2170/pone.0168885.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8ce/5173245/ad2caa5190ea/pone.0168885.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8ce/5173245/45b298127d20/pone.0168885.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8ce/5173245/67be81ec8c06/pone.0168885.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8ce/5173245/f16e325d1705/pone.0168885.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8ce/5173245/46c16d434b7d/pone.0168885.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8ce/5173245/348c3bd8f23a/pone.0168885.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8ce/5173245/7a0bf25a2170/pone.0168885.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8ce/5173245/ad2caa5190ea/pone.0168885.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8ce/5173245/45b298127d20/pone.0168885.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8ce/5173245/67be81ec8c06/pone.0168885.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8ce/5173245/f16e325d1705/pone.0168885.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8ce/5173245/46c16d434b7d/pone.0168885.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8ce/5173245/348c3bd8f23a/pone.0168885.g007.jpg

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